ArticleThe Journal of cell biology2014
The nebulin repeat protein Lasp regulates I-band architecture and filament spacing in myofibrils.
Article in The Journal of cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Developmental remodelling ofOpen biology · 2025Article
- The oxoglutarate dehydrogenase complex is involved in myofibril growth and Z-disc assembly in Drosophila.Journal of cell science · 2023Article
- Drosophila Tropomodulin is required for multiple actin-dependent processes within developing myofibers.Development (Cambridge, England) · 2023Article
- Independent pathways control muscle tissue size and sarcomere remodeling.Developmental biology · 2022Article
- The Mechanisms of Thin Filament Assembly and Length Regulation in Muscles.International journal of molecular sciences · 2022Review
- Review
- Analyzing muscle structure and function throughout the larval instars in liveSTAR protocols · 2021Article
- Natural variation in the regulation of neurodevelopmental genes modifies flight performance in Drosophila.PLoS genetics · 2021Article
- The actin polymerization factor Diaphanous and the actin severing protein Flightless I collaborate to regulate sarcomere size.Developmental biology · 2021Article
- Organelle size scaling over embryonic development.Wiley interdisciplinary reviews. Developmental biology · 2020Review
- Cofilin Loss in Drosophila Muscles Contributes to Muscle Weakness through Defective Sarcomerogenesis during Muscle Growth.Cell reports · 2020Article
- Review
- Bimolecular Fluorescence Complementation (BiFC) for Studying Sarcomeric Protein Interactions inBio-protocol · 2020Article
- Comprehensive analysis of the cardiac proteome in a rat model of myocardial ischemia-reperfusion using a TMT-based quantitative proteomic strategy.Proteome science · 2020Article
- Article
- TheThe Journal of cell biology · 2019Article
- Article
- Filamin actin-binding and titin-binding fulfill distinct functions in Z-disc cohesion.PLoS genetics · 2017Article
- Overview of the Muscle Cytoskeleton.Comprehensive Physiology · 2017Review
- TheeLife · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mutations in nebulin, a giant muscle protein with 185 actin-binding nebulin repeats, are the major cause of nemaline myopathy in humans. Nebulin sets actin thin filament length in sarcomeres, potentially by stabilizing thin filaments in the I-band, where nebulin and thin filaments coalign. However, the precise role of nebulin in setting thin filament length and its other functions in regulating power output are unknown. Here, we show that Lasp, the only member of the nebulin family in Drosophila melanogaster, acts at two distinct sites in the sarcomere and controls thin filament length with just two nebulin repeats. We found that Lasp localizes to the Z-disc edges to control I-band architecture and also localizes at the A-band, where it interacts with both actin and myosin to set proper filament spacing. Furthermore, introducing a single amino acid change into the two nebulin repeats of Lasp demonstrated different roles for each domain and established Lasp as a suitable system for studying nebulin repeat function.
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Registered trials
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