ArticleThe Journal of biological chemistry2014
The role of constitutive nitric-oxide synthase in ultraviolet B light-induced nuclear factor κB activity.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Ultraviolet (UV) radiation: a double-edged sword in cancer development and therapy.Molecular biomedicine · 2024Review
- Characterization of UVB and UVA-340 Lamps and Determination of Their Effects on ER Stress and DNA Damage.Photochemistry and photobiology · 2022Article
- UVB-induced eIF2α phosphorylation in keratinocytes depends on decreased ATF4, GADD34 and CReP expression levels.Life sciences · 2021Article
- Ultraviolet Radiation and Basal Cell Carcinoma: An Environmental Perspective.Frontiers in public health · 2021Review
- Role of constitutive nitric oxide synthases in the dynamic regulation of the autophagy response of keratinocytes upon UVB exposure.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2020Article
- Anticancer Activity ofNutrients · 2020Review
- Potential Immunoregulatory and Antiviral/SARS-CoV-2 Activities of Nitric Oxide.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- PM2.5 exposure induces vascular dysfunction via NO generated by iNOS in lung of ApoE-/- mouse.International journal of biological sciences · 2020Article
- The Effect of Endothelial Cells on UVB-induced DNA Damage and Transformation of Keratinocytes In 3D Polycaprolactone Scaffold Co-culture System.Photochemistry and photobiology · 2019Article
- The Mechanisms of Carnosol in Chemoprevention of Ultraviolet B-Light-Induced Non-Melanoma Skin Cancer Formation.Scientific reports · 2018Article
- Antitumoral gene-based strategy involving nitric oxide synthase type III overexpression in hepatocellular carcinoma.Gene therapy · 2016Article
- Ultraviolet radiation-induced non-melanoma skin cancer: Regulation of DNA damage repair and inflammation.Genes & diseases · 2014Article
- The role of constitutive nitric-oxide synthase in regulation of IKKα after ultraviolet irradiation.Photochemistry and photobiologyArticle
- Constitutive nitric oxide synthases deficiency impairs cyclobutane pyrimidine dimer repair following solar UV exposure in cells and mice.Photochemistry and photobiologyArticle
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
NF-κB is a transcription factor involved in many signaling pathways that also plays an important role in UV-induced skin tumorigenesis. UV radiation can activate NF-κB, but the detailed mechanism remains unclear. In this study, we provided evidence that the activation of constitutive nitric-oxide synthase plays a role in regulation of IκB reduction and NF-κB activation in human keratinocyte HaCaT cells in early phase (within 6 h) post-UVB. Treating the cells with l-NAME, a selective inhibitor of constitutive nitric-oxide synthase (cNOS), can partially reverse the IκB reduction and inhibit the DNA binding activity as well as nuclear translocation of NF-κB after UVB radiation. A luciferase reporter assay indicates that UVB-induced NF-κB activation is totally diminished in cNOS null cells. The cNOS-mediated reduction of IκB is likely due to the imbalance of nitric oxide/peroxynitrite because treating the cells with lower (50 μm), but not higher (100-500 μm), concentration of S-nitroso-N-acetylpenicillamine (SNAP) can reverse the effect of l-NAME in partial restore IκB level post-UVB. Our data also showed that NF-κB activity was required for maintaining a stable IκB kinase α subunit (IKKα) level because treating the cells with NF-κB or cNOS inhibitors could reduce IKKα level upon UVB radiation. In addition, our data demonstrated that although NF-κB protects cells from UVB-induced death, its pro-survival activity was likely neutralized by the pro-death activity of peroxynitrite after UVB radiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.