ArticleBMC genomics2014
De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla, a non-model plant with potent anti-alzheimer's properties.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 75 citations in OpenAlex.
- Genome-Wide Identification and Expression Analysis of the U-Box E3 Ubiquitin Ligase Gene Family Related to Monoterpene Indole Alkaloids Biosynthesis inInternational journal of molecular sciences · 2026Article
- The genome of the medicinal plant Uncaria rhynchophylla provides new insights into monoterpenoid indole alkaloid metabolism and its molecular regulatory mechanism.Molecular horticulture · 2026Article
- Review
- GWAS Reveals Key Candidate Genes Associated with Milk-Production in Saanen Goats.Animals : an open access journal from MDPI · 2025Article
- Identification and Functional Characterization of a Geraniol Synthase UrGES fromPlants (Basel, Switzerland) · 2025Article
- A sterile plant culture system ofPlant biotechnology (Tokyo, Japan) · 2025Article
- Induction of Monoterpenoid Oxindole Alkaloids Production and Related Biosynthetic Gene Expression in Response to Signaling Molecules inPlants (Basel, Switzerland) · 2024Article
- Genome-wide identification of GATA transcription factor family and the effect of different light quality on the accumulation of terpenoid indole alkaloids in Uncaria rhynchophylla.Plant molecular biology · 2024Article
- The beneficial pharmacological effects ofFrontiers in pharmacology · 2024Review
- Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns ofInternational journal of molecular sciences · 2023Article
- Profiles of Metabolic Genes inBiomolecules · 2022Article
- Transcriptome revealing the dual regulatory mechanism of ethylene on the rhynchophylline and isorhynchophylline in Uncaria rhynchophylla.Journal of plant research · 2022Article
- Comparative transcriptome analysis revealed the molecular mechanism of the effect of light intensity on the accumulation of rhynchophylline and isorhynchophylline inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Computer Aided Drug Design Methodologies with Natural Products in the Drug Research Against Alzheimer's Disease.Current neuropharmacology · 2022Review
- Recent Advances in the Anti-Inflammatory Activity of Plant-Derived Alkaloid Rhynchophylline in Neurological and Cardiovascular Diseases.Pharmaceutics · 2021Review
- Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal inFrontiers in plant science · 2021Article
- A comparative analysis of gene expression induced by the embryo in the caprine endometrium.Veterinary medicine and science · 2020Article
- Analyses of circRNA profiling during the development from pre-receptive to receptive phases in the goat endometrium.Journal of animal science and biotechnology · 2019Article
- Transcriptome analysis of maca (Applications in plant sciences · 2018Article
- Transcriptional profiles of crossbred embryos derived from yak oocytes in vitro fertilized with cattle sperm.Scientific reports · 2018Article
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Authors and funding
10 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe major medicinal alkaloids isolated from Uncaria rhynchophylla (gouteng in chinese) capsules are rhynchophylline (RIN) and isorhynchophylline (IRN). Extracts containing these terpene indole alkaloids (TIAs) can inhibit the formation and destabilize preformed fibrils of amyloid β protein (a pathological marker of Alzheimer's disease), and have been shown to improve the cognitive function of mice with Alzheimer-like symptoms. The biosynthetic pathways of RIN and IRN are largely unknown.
resultsIn this study, RNA-sequencing of pooled Uncaria capsules RNA samples taken at three developmental stages that accumulate different amount of RIN and IRN was performed. More than 50 million high-quality reads from a cDNA library were generated and de novo assembled. Sequences for all of the known enzymes involved in TIAs synthesis were identified. Additionally, 193 cytochrome P450 (CYP450), 280 methyltransferase and 144 isomerase genes were identified, that are potential candidates for enzymes involved in RIN and IRN synthesis. Digital gene expression profile (DGE) analysis was performed on the three capsule developmental stages, and based on genes possessing expression profiles consistent with RIN and IRN levels; four CYP450s, three methyltransferases and three isomerases were identified as the candidates most likely to be involved in the later steps of RIN and IRN biosynthesis.
conclusionA combination of de novo transcriptome assembly and DGE analysis was shown to be a powerful method for identifying genes encoding enzymes potentially involved in the biosynthesis of important secondary metabolites in a non-model plant. The transcriptome data from this study provides an important resource for understanding the formation of major bioactive constituents in the capsule extract from Uncaria, and provides information that may aid in metabolic engineering to increase yields of these important alkaloids.
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