ArticleBiomacromolecules2014
Hybrid elastin-like polypeptide-polyethylene glycol (ELP-PEG) hydrogels with improved transparency and independent control of matrix mechanics and cell ligand density.
Article in Biomacromolecules, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- Biomimetic peptide conjugates as emerging strategies for controlled release from protein-based materials.Drug delivery · 2025Review
- Modeling Uterine Fibroids Using Bioengineered Hydrogels.ACS biomaterials science & engineering · 2025Article
- Submucosal Hydrogel for Spring-Mediated Intestinal Lengthening.Journal of biomedical materials research. Part A · 2025Article
- Advances in hybrid hydrogel design for biomedical applications: innovations in drug delivery and tissue engineering for gynecological cancers.Cell biology and toxicology · 2025Review
- The Optimization of Culture Conditions for Injectable Recombinant Collagen Hydrogel Preparation Using Machine Learning.Gels (Basel, Switzerland) · 2025Article
- Evaluation of Articular Cartilage Regeneration Properties of Decellularized Cartilage Powder/Modified Hyaluronic Acid Hydrogel Scaffolds.ACS omega · 2024Article
- Supramolecular assembly of multifunctional protein gels via anMolecular systems design & engineering · 2024Article
- Molecular co-assembled strategy tuning protein conformation for cartilage regeneration.Nature communications · 2024Article
- Exploring stimuli-responsive elastin-like polypeptide for biomedicine and beyond: potential application as programmable soft actuators.Frontiers in bioengineering and biotechnology · 2023Review
- Tuning Polymer Hydrophilicity to Regulate Gel Mechanics and Encapsulated Cell Morphology.Advanced healthcare materials · 2022Article
- Facile Fabrication of Transparent and Opaque Albumin Methacryloyl Gels with Highly Improved Mechanical Properties and Controlled Pore Structures.Gels (Basel, Switzerland) · 2022Article
- Elastin-like Proteins to Support Peripheral Nerve Regeneration in Guidance Conduits.ACS biomaterials science & engineering · 2021Article
- Application of Thermoresponsive Intrinsically Disordered Protein Polymers in Nanostructured and Microstructured Materials.Macromolecular bioscience · 2021Review
- Reconstructing the heart using iPSCs: Engineering strategies and applications.Journal of molecular and cellular cardiology · 2021Review
- Advanced hydrogels for the repair of cartilage defects and regeneration.Bioactive materials · 2021Review
- Bio-orthogonally crosslinked hyaluronate-collagen hydrogel for suture-free corneal defect repair.Biomaterials · 2020Article
- Engineering the Architecture of Elastin-Like Polypeptides: From Unimers to Hierarchical Self-Assembly.Advanced therapeutics · 2020Article
- Digging deeper: structural background of PEGylated fibrin gels in cell migration and lumenogenesis.RSC advances · 2020Article
- Review
- Engineered materials for organoid systems.Nature reviews. Materials · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Hydrogels have been developed as extracellular matrix (ECM) mimics both for therapeutic applications and basic biological studies. In particular, elastin-like polypeptide (ELP) hydrogels, which can be tuned to mimic several biochemical and physical characteristics of native ECM, have been constructed to encapsulate various types of cells to create in vitro mimics of in vivo tissues. However, ELP hydrogels become opaque at body temperature because of ELP's lower critical solution temperature behavior. This opacity obstructs light-based observation of the morphology and behavior of encapsulated cells. In order to improve the transparency of ELP hydrogels for better imaging, we have designed a hybrid ELP-polyethylene glycol (PEG) hydrogel system that rapidly cross-links with tris(hydroxymethyl) phosphine (THP) in aqueous solution via Mannich-type condensation. As expected, addition of the hydrophilic PEG component significantly improves the light transmittance. Coherent anti-Stokes Raman scattering (CARS) microscopy reveals that the hybrid ELP-PEG hydrogels have smaller hydrophobic ELP aggregates at 37 °C. Importantly, this hydrogel platform enables independent tuning of adhesion ligand density and matrix stiffness, which is desirable for studies of cell-matrix interactions. Human fibroblasts encapsulated in these hydrogels show high viability (>98%) after 7 days of culture. High-resolution confocal microscopy of encapsulated fibroblasts reveals that the cells adopt a more spread morphology in response to higher RGD ligand concentrations and softer gel mechanics.
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Registered trials
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