ArticlePloS one2014
The blast resistance gene Pi54of cloned from Oryza officinalis interacts with Avr-Pi54 through its novel non-LRR domains.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed.
- Genome-wide prediction of cloned disease resistance homologues across the Oryza genus.The plant genome · 2026Article
- A successful stacking of multiple biotic stress resistance in rice facilitated by genes and precision breeding tools.Biological research · 2026Article
- Cloning ofPlants (Basel, Switzerland) · 2026Article
- Research Progress on Rice-Blast-Resistance-Related Genes.Plants (Basel, Switzerland) · 2025Review
- A century of advances in molecular genetics and breeding for sustainable resistance to rice blast disease.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Improving the cultivated rice Sakha104 (PeerJ · 2025Article
- Magnaporthe oryzae effector AvrPik-D targets a transcription factor WG7 to suppress rice immunity.Rice (New York, N.Y.) · 2024Article
- Recent Advances in Effector Research ofBiomolecules · 2023Review
- iTRAQ based proteomic analysis of rice lines having single or stacked blast resistance genes:Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Approaches to Reduce Rice Blast Disease Using Knowledge from Host Resistance and Pathogen Pathogenicity.International journal of molecular sciences · 2023Review
- Endophytic bacterial communities in wild rice (Frontiers in plant science · 2023Article
- Biological control ofFrontiers in microbiology · 2023Article
- Wheat blast: The last enemy of hunger fighters.Genetics and molecular biology · 2023Article
- Advances in Fungal Elicitor-Triggered Plant Immunity.International journal of molecular sciences · 2022Review
- Advancement in the Breeding, Biotechnological and Genomic Tools towards Development of Durable Genetic Resistance against the Rice Blast Disease.Plants (Basel, Switzerland) · 2022Review
- Understanding the Dynamics of Blast Resistance in Rice-Journal of fungi (Basel, Switzerland) · 2022Review
- Thirty years of resistance: Zig-zag through the plant immune system.The Plant cell · 2022Review
- Interspecific Hybridization Is an Important Driving Force for Origin and Diversification of Asian Cultivated RiceFrontiers in plant science · 2022Review
- Computational models for prediction of protein-protein interaction in rice andFrontiers in plant science · 2022Article
- Arms and ammunitions: effectors at the interface of rice and it's pathogens and pests.Rice (New York, N.Y.) · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dominant rice blast resistance gene Pi54 cloned by map-based cloning approach from indica rice cultivar Tetep confers broad spectrum resistance to Magnaporthe oryzae. In this investigation, an orthologue of Pi54 designated as Pi54of was cloned from Oryza officinalis conferring high degree of resistance to M. oryzae and is functionally validated. We have also characterized the Pi54of protein and demonstrate its interaction with AVR-Pi54 protein. The Pi54of encoded ∼43 kDa small and unique cytoplasmic LRR family of disease resistance protein having unique Zinc finger domain overlapped with the leucine rich repeat regions. Pi54of showed Magnaporthe-induced expression. The phylogenetic and western blot analysis confirmed orthologous nature of Pi54 and Pi54of genes, whereas the identity of protein was confirmed through MALDI-TOF analysis. The in silico analysis showed that Pi54of is structurally more stable than other cloned Pi54 proteins. The molecular docking revealed that Pi54of protein interacts with AVR-Pi54 through novel non-LRR domains such as STI1 and RhoGEF. The STI1 and GEF domains which interact with AVR-Pi54 are also components of rice defensome complex. The Pi54of protein showed differential domain specificity while interacting with the AVR protein. Functional complementation revealed that Pi54of transferred in two rice lines belonging to indica and japonica background imparts enhanced resistance against three highly virulent strains of M. oryzae. In this study, for the first time, we demonstrated that a rice blast resistance gene Pi54of cloned from wild species of rice provides high degree of resistance to M. oryzae and might display different molecular mechanism involved in AVRPi54-Pi54of interaction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.