ArticleBioMed research international2014
Preparation of three-dimensional vascularized MSC cell sheet constructs for tissue regeneration.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Three-dimensional cell sheet model improvesBioactive materials · 2025Article
- Scaffold-free 3D culture systems for stem cell-based tissue regeneration.APL bioengineering · 2024Review
- Tailoring cell sheets for biomedical applications.Smart medicine · 2024Review
- Application of mesenchymal stem cell sheet for regeneration of craniomaxillofacial bone defects.Stem cell research & therapy · 2023Review
- An efficient method for cell sheet bioengineering from rBMSCs on thermo-responsive PCL-PEG-PCL copolymer.Journal of biological engineering · 2023Article
- Osteogenesis in human periodontal ligament stem cell sheets is enhanced by the protease-activated receptor 1 (PARScientific reports · 2022Article
- Vascularization strategies in tissue engineering approaches for soft tissue repair.Journal of tissue engineering and regenerative medicine · 2021Review
- Mechanistic Illustration: How Newly-Formed Blood Vessels Stopped by the Mineral Blocks of Bone Substitutes Can Be Avoided by Using Innovative Combined Therapeutics.Biomedicines · 2021Article
- Measurement of the Adipose Stem Cells Cell Sheets Transmittance.Bioengineering (Basel, Switzerland) · 2021Article
- Novel pre-vascularized tissue-engineered dermis based on stem cell sheet technique used for dermis-defect healing.Regenerative biomaterials · 2020Article
- Circular RNA-ABCB10 promotes angiogenesis induced by conditioned medium from human amnion-derived mesenchymal stem cells via the microRNA-29b-3p/vascular endothelial growth factor A axis.Experimental and therapeutic medicine · 2020Article
- [Construction of three-dimensional dermoid tissue based on cell sheets technologyZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020Article
- Circ-100290 Positively Regulates Angiogenesis Induced by Conditioned Medium of Human Amnion-Derived Mesenchymal Stem Cells Through miR-449a/eNOS and miR-449a/VEGFA Axes.International journal of biological sciences · 2020Article
- Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review.Cell & bioscience · 2020Review
- Tissue Engineering and Regenerative Medicine: Achievements, Future, and Sustainability in Asia.Frontiers in bioengineering and biotechnology · 2020Review
- Bioactive factors for cartilage repair and regeneration: Improving delivery, retention, and activity.Acta biomaterialia · 2019Review
- Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application.International journal of oral science · 2019Review
- Biofabrication strategies for creating microvascular complexity.Biofabrication · 2019Review
- Unveiling Mesenchymal Stromal Cells' Organizing Function in Regeneration.International journal of molecular sciences · 2019Review
- Endothelial progenitor cells improve the therapeutic effect of mesenchymal stem cell sheets on irradiated bone defect repair in a rat model.Journal of translational medicine · 2018Article
Corrections and comments
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Authors and funding
7 authors.
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Abstract
Engineering three-dimensional (3D) vascularized constructs remains a challenge due to the inability to form rich microvessel networks. In this study we engineered a prevascularized 3D cell sheet construct for tissue regeneration using human bone marrow-derived mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells as cell sources. hMSCs were cultured to form a thick cell sheet, and human umbilical vein endothelial cells (HUVECs) were then seeded on the hMSCs sheet to form networks. The single prevascularized HUVEC/hMSC cell sheet was folded to form a 3D construct by a modified cell sheet engineering technique. In vitro results indicated that the hMSCs cell sheet promoted the HUVECs cell migration to form networks in horizontal and vertical directions. In vivo results showed that many blood vessels grew into the 3D HUVEC/hMSC cell sheet constructs after implanted in the subcutaneous pocket of immunodeficient mice. The density of blood vessels in the prevascularized constructs was higher than that in the nonprevascularized constructs. Immunohistochemistry staining further showed that in vitro preformed human capillaries in the prevascularized constructs anastomosed with the host vasculature to form functional blood vessels. These results suggest the promising potential of this 3D prevascularized construct using hMSCs cell sheet as a platform for wide applications in engineering vascularized tissues.
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