ArticleNature materials2014
Interplay of matrix stiffness and protein tethering in stem cell differentiation.
Article in Nature materials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 405 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
405 citing papers in PubMed, 1 synthesis or guideline pooled it.
- High throughput interrogation of human liver stellate cells reveals microenvironmental regulation of phenotype.Acta biomaterialia · 2022Pooled it
- Mechano-immunomodulatory biomaterials: From immune mechanosensing to translational design.Bioactive materials · 2026Review
- Extracellular matrix composition and stiffness regulates progesterone responsiveness and decidualization.iScience · 2026Article
- Strategies for the Selection and Application of Biological Scaffolds in Organ-on-a-Chip Systems.Chembiochem : a European journal of chemical biology · 2026Review
- Mechanical Signaling in the Microenvironment Regulates the Differentiation of Dental Pulp Stem Cells: A Novel Strategy For Pulp-Dentin Complex Regeneration.Stem cell reviews and reports · 2026Review
- Glycosaminoglycan-functionalized hydrogels for sustained delivery of tissue inhibitor of metalloproteinase-3 mediating matrix metalloprotease inhibition and extracellular matrix stabilization.Bioactive materials · 2026Article
- Modeling mechanochemical coupling in optogenetically activated cell layers.Biophysical journal · 2026Article
- Microengineered Gradient Hydrogels for Mechanobiology.Advanced healthcare materials · 2026Review
- Ribbon-shaped microgels as bioinks for 3D bioprinting of anisotropic tissue structures.Bioactive materials · 2026Article
- Research on the regulation of osteogenic differentiation and mechanotransduction by porous bionic coatings on titanium alloy implant surfaces.RSC advances · 2026Article
- Fluorescently Labeled Gradient Hydrogels Reveal Matrix-Dependent Cell Responses to Substrate Stiffness.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Mechanical rejuvenation of senescent stem cells and aged bone via chromatin remodeling.Nature communications · 2026Article
- Effect of 2D and 3D ECM and Biomechanical Cues on Human iPSC-Derived Liver Progenitor Cell Differentiation.Advanced healthcare materials · 2026Article
- Extracellular Matrix Microstructures Directly Regulate Glutathione Bioavailability in Human Hepatocytes.Biomacromolecules · 2025Article
- Bioinspired honeycomb-mimetic adaptive hyaluronic acid hydrogel composite scaffold: intelligent prevention and treatment of infection of bone implants and promotion of bone repair.Materials today. Bio · 2025Article
- Mechanical regulation of N2A neural cell behavior by microtissue densification.In vitro cellular & developmental biology. Animal · 2025Article
- Open Challenges and Opportunities in Piezoelectricity for Tissue Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Integrated bioprinting of trachea-like structures based on tissue-specific bioink.Materials today. Bio · 2025Article
- 3D traction force microscopy in human trabecular meshwork tissues: Effects of ROCK and YAP/TAZ inhibition in normal and glaucomatous tissues.Tissue & cell · 2025Article
- Matrix directs trophoblast differentiation in a bioprinted organoid model of early placental development.Nature communications · 2025Article
345 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Stem cells regulate their fate by binding to, and contracting against, the extracellular matrix. Recently, it has been proposed that in addition to matrix stiffness and ligand type, the degree of coupling of fibrous protein to the surface of the underlying substrate, that is, tethering and matrix porosity, also regulates stem cell differentiation. By modulating substrate porosity without altering stiffness in polyacrylamide gels, we show that varying substrate porosity did not significantly change protein tethering, substrate deformations, or the osteogenic and adipogenic differentiation of human adipose-derived stromal cells and marrow-derived mesenchymal stromal cells. Varying protein-substrate linker density up to 50-fold changed tethering, but did not affect osteogenesis, adipogenesis, surface-protein unfolding or underlying substrate deformations. Differentiation was also unaffected by the absence of protein tethering. Our findings imply that the stiffness of planar matrices regulates stem cell differentiation independently of protein tethering and porosity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.