ArticleBrain stimulation
Seizure suppression by high frequency optogenetic stimulation using in vitro and in vivo animal models of epilepsy.
Article in Brain stimulation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 67 citations in OpenAlex.
- Article
- Nanophotonic neural probes for in vivo photostimulation, electrophysiology, and microfluidic delivery.Microsystems & nanoengineering · 2026Article
- Nonlinear dynamics of seizure suppression via optogenetic modulation of neuron-astrocyte interaction.Scientific reports · 2026Article
- Thermally Drawn Multifunctional All-Hydrogel Fibers for Anti-Fibrotic and Multimodal Neural Interfaces.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Enhanced Distal Signaling in Human Hippocampal Neurons despite Lower Intrinsic Excitability.Research square · 2025Article
- Cell-type-specific networks during hippocampal seizures at the micro- and macroscale.Brain : a journal of neurology · 2025Article
- Micro-Coil Neuromodulation at Single-Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- On brain stimulation in epilepsy.Brain : a journal of neurology · 2025Review
- Delta-fast ripple coupling suppression: designing a brain-mimetic stimulation paradigm for seizure abolishment.Frontiers in neuroscience · 2025Article
- Recent advances and current status of gene therapy for epilepsy.World journal of pediatrics : WJP · 2024Review
- A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level.Nature communications · 2024Article
- Neurophotonic methods in approach to in vivo animal epileptic models: Advantages and limitations.Epilepsia · 2024Review
- Optogenetic spinal stimulation promotes new axonal growth and skilled forelimb recovery in rats with sub-chronic cervical spinal cord injury.Journal of neural engineering · 2023Article
- A Comprehensive Review of Emerging Trends and Innovative Therapies in Epilepsy Management.Brain sciences · 2023Review
- Involvement of GABAergic Interneuron Subtypes in 4-Aminopyridine-Induced Seizure-Like Events in Mouse Entorhinal CortexThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- In vivo inhibition of epileptiform afterdischarges in rat hippocampus by light-activated chloride channel, stGtACR2.CNS neuroscience & therapeutics · 2023Article
- LINCs Are Vulnerable to Epileptic Insult and Fail to Provide Seizure Control via On-Demand Activation.eNeuro · 2023Article
- Using Constrained Square-Root Cubature Kalman Filter for Quantifying the Severity of Epileptic Activities in Mice.Biomedicines · 2022Article
- Electrical stimulation of the endopiriform nucleus attenuates epilepsy in rats by network modulation.Annals of clinical and translational neurology · 2020Article
- Neural mass modeling of slow-fast dynamics of seizure initiation and abortion.PLoS computational biology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundElectrical high frequency stimulation (HFS) has been shown to suppress seizures. However, the mechanisms of seizure suppression remain unclear and techniques for blocking specific neuronal populations are required.
objectiveThe goal is to study the optical HFS protocol on seizures as well as the underlying mechanisms relevant to the HFS-mediated seizure suppression by using optogenetic methodology.
methodsThy1-ChR2 transgenic mice were used in both vivo and in vitro experiments. Optical stimulation with pulse trains at 20 and 50 Hz was applied on the focus to determine its effects on in vivo seizure activity induced by 4-AP and recorded in the bilateral and ipsilateral-temporal hippocampal CA3 regions. In vitro methodology was then used to study the mechanisms of the in vivo suppression.
resultsOptical HFS was able to generate 82.4% seizure suppression at 50 Hz with light power of 6.1 mW and 80.2% seizure suppression at 20 Hz with light power of 2.0 mW. The suppression percentage increased by increasing the light power and saturated when the power reached above-mentioned values. In vitro experimental results indicate that seizure suppression was mediated by activation of GABA receptors. Seizure suppression effect decreased with continued application but the suppression effect could be restored by intermittent stimulation.
conclusionsThis study shows that optical stimulation at high frequency targeting an excitatory opsin has potential therapeutic application for fast control of an epileptic focus. Furthermore, electrophysiological observations of extracellular and intracellular signals revealed that GABAergic neurotransmission activated by optical stimulation was responsible for the suppression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.