ArticlePlant molecular biology2014
A novel method to identify the DNA motifs recognized by a defined transcription factor.
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 50 citations in OpenAlex.
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- Genome-Wide Analysis of Heat Shock Transcription Factors (HSFs) in Kelp (Plants (Basel, Switzerland) · 2026Article
- From binding to networks: methods for identifying transcription factor targets in plant systems.Frontiers in plant science · 2026Review
- Viral proteins suppress rice defenses by boosting OsTSN1 RNA decay via phase separation and multimerization.Nature communications · 2025Article
- Integrated transcriptomic, transcriptional factors, and protein interaction reveal the regulatory mechanisms of flowering time in rice (Oryza sativa L.).Transgenic research · 2025Article
- Workflow to Select Functional Promoter DNA Baits and Screen Arrayed Gene Libraries in Yeast.Current protocols · 2024Article
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- Building an improved transcription factor-centered yeast one hybrid system to identify DNA motifs bound by protein comprehensively.BMC plant biology · 2023Article
- SlZF3 regulates tomato plant height by directly repressingHorticulture research · 2023Article
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- The PagKNAT2/6b-PagBOP1/2a Regulatory Module Controls Leaf Morphogenesis inInternational journal of molecular sciences · 2022Article
- UNFERTILIZED EMBRYO SAC 12 phosphorylation plays a crucial role in conferring salt tolerance.Plant physiology · 2022Article
- RLM1, Encoding an R2R3 MYB Transcription Factor, Regulates the Development of Secondary Cell Wall in Rice.Frontiers in plant science · 2022Article
- A systems biology approach identifies a regulator,Forestry research · 2021Article
- A R2R3-MYB Transcription Factor Gene,Frontiers in plant science · 2021Article
- Overexpression ofInternational journal of molecular sciences · 2019Article
- Transcription Factor ANAC074 Binds to NRS1, NRS2, or MybSt1 Element in Addition to the NACRS to Regulate Gene Expression.International journal of molecular sciences · 2018Article
- A Novel R2R3-MYB Transcription Factor BpMYB106 of Birch (Betula platyphylla) Confers Increased Photosynthesis and Growth Rate through Up-regulating Photosynthetic Gene Expression.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction between a protein and DNA is involved in almost all cellular functions, and is vitally important in cellular processes. Two complementary approaches are used to detect the interactions between a transcription factor (TF) and DNA, i.e. the TF-centered or protein-DNA approach, and the gene-centered or DNA-protein approach. The yeast one-hybrid (Y1H) is a powerful and widely used system to identify DNA-protein interactions. However, a powerful method to study protein-DNA interactions like Y1H is lacking. Here, we developed a protein-DNA method based on the Y1H system to identify the motifs recognized by a defined TF, termed TF-centered Y1H. In this system, a random short DNA sequence insertion library was generated as the prey DNA sequences to interact with a defined TF as the bait. Using this system, novel interactions were detected between DNA motifs and the AtbZIP53 protein from Arabidopsis. We identified six motifs that were specifically bound by AtbZIP53, including five known motifs (DOF, G-box, I-box, BS1 and MY3) and a novel motif BRS1 [basic leucine zipper (bZIP) Recognized Site 1]. The different subfamily bZIP members also recognize these six motifs, further confirming the reliability of the TF-centered Y1H results. Taken together, these results demonstrated that TF-centered Y1H could identify quickly the motifs bound by a defined TF, representing a reliable and efficient approach with the advantages of Y1H. Therefore, this TF-centered Y1H may have a wide application in protein-DNA interaction studies.
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