ArticleCell stress & chaperones2015
Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells.
Article in Cell stress & chaperones, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Response of mid-lactation primiparous Holstein cows to the supplementation of rumen-protected methionine during the summer.PloS one · 2026Article
- Effects of Dietary Rumen-Protected Choline on Stress Alleviation, Antioxidant Modulation, and Haematological, Immunological, and Offspring Performance in Transitioning Goats.Veterinary medicine and science · 2025Article
- High water temperature raised the requirements of methionine for spotted seabass (Lateolabrax maculatus).Fish physiology and biochemistry · 2024Article
- Effects of Rumen-Protected L-Tryptophan Supplementation on Productivity, Physiological Indicators, Blood Profiles, and Heat Shock Protein Gene Expression in Lactating Holstein Cows under Heat Stress Conditions.International journal of molecular sciences · 2024Article
- Varying the ratio of Lys: Met through enhancing methionine supplementation improved milk secretion ability through regulating the mRNA expression in bovine mammary epithelial cells under heat stress.Frontiers in veterinary science · 2024Article
- Enhancing bovine immune, antioxidant and anti-inflammatory responses with vitamins, rumen-protected amino acids, and trace minerals to prevent periparturient mastitis.Frontiers in immunology · 2023Review
- Effects of PUFA-Rich Dietary Strategies on Ruminants' Mammary Gland Gene Network: A Nutrigenomics Review.Metabolites · 2022Review
- Increased supply of methionine during a heat-stress challenge in lactating holstein cows alters mammary tissue mTOR signaling and its response to lipopolysaccharide.Journal of animal science · 2022Article
- Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells.International journal of molecular sciences · 2022Article
- In vitro effects of 5-Hydroxy-L-tryptophan supplementation on primary bovine mammary epithelial cell gene expression under thermoneutral or heat shock conditions.Scientific reports · 2022Article
- Overview of the effect of rumen-protected limiting amino acids (methionine and lysine) and choline on the immunity, antioxidative, and inflammatory status of periparturient ruminants.Frontiers in immunology · 2022Review
- Immune and metabolic effects of rumen-protected methionine during a heat stress challenge in lactating Holstein cows.Journal of animal science · 2021Article
- Enhanced supply of methionine regulates protein synthesis in bovine mammary epithelial cells under hyperthermia condition.Journal of animal science and technology · 2021Article
- Effect of incubation temperature on lactogenic function of goat milk-derived mammary epithelial cells.In vitro cellular & developmental biology. Animal · 2020Article
- Transcriptome Functional Analysis of Mammary Gland of Cows in Heat Stress and Thermoneutral Condition.Animals : an open access journal from MDPI · 2020Article
- Dietary Nutrients and Bioactive Substances Modulate Heat Shock Protein (HSP) Expression: A Review.Nutrients · 2018Review
- Article
- The global effect of heat on gene expression in cultured bovine mammary epithelial cells.Cell stress & chaperones · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to investigate the effects of methionine on cell proliferation, antioxidant activity, apoptosis, the expression levels of related genes (HSF-1, HSP70, Bax and Bcl-2) and the expression levels of protein (HSP70) in mammary epithelial cells, after heat treatment. Methionine (60 mg/L) increased the viability and attenuated morphological damage in hyperthermia-treated bovine mammary epithelial cells (BMECs). Additionally, methionine significantly reduced lactate dehydrogenase leakage, malondialdehyde formation, nitric oxide, and nitric oxide synthase activity. Superoxide dismutase, catalase, and glutathione peroxidase enzymatic activity was increased significantly in the presence of methionine. Bovine mammary epithelial cells also exhibited a certain amount of HSP70 reserve after methionine pretreatment for 24 h, and the expression level of the HSP70 gene and protein further increased with incubation at 42 °C for 30 min. Compared to the control, the expression of HSF-1 mRNA increased, and there was a significantly reduced expression of Bax/Bcl-2 mRNA and a reduced activity of caspase-3 against heat stress. Methionine also increased survival and decreased early apoptosis of hyperthermia-treated BMECs. Thus, methionine has cytoprotective effects on hyperthermia-induced damage in BMECs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.