ArticleNature structural & molecular biology2014
EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon-anticodon duplex.
Article in Nature structural & molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 44 citations in OpenAlex.
- Structure-function comparison of Arbekacin with other aminoglycosides elucidates its higher potency as bacterial translation inhibitor.Scientific reports · 2025Article
- Paenilamicins are context-specific translocation inhibitors of protein synthesis.Nature chemical biology · 2024Article
- Tuning tRNAs for improved translation.Frontiers in genetics · 2024Review
- Genome Expansion by tRNA +1 Frameshifting at Quadruplet Codons.Journal of molecular biology · 2022Review
- Genetic code degeneracy is established by the decoding center of the ribosome.Nucleic acids research · 2022Article
- Translational Control of COVID-19 and Its Therapeutic Implication.Frontiers in immunology · 2022Review
- Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP.Nature communications · 2021Article
- Ribosome as a Translocase and Helicase.Biochemistry. Biokhimiia · 2021Review
- Structural basis for +1 ribosomal frameshifting during EF-G-catalyzed translocation.Nature communications · 2021Article
- Mechanistic insights into translation inhibition by aminoglycoside antibiotic arbekacin.Nucleic acids research · 2021Article
- Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA.Nature communications · 2021Article
- Multifaceted Mechanism of Amicoumacin A Inhibition of Bacterial Translation.Frontiers in microbiology · 2021Article
- Mutations in domain IV of elongation factor EF-G confer -1 frameshifting.RNA (New York, N.Y.) · 2021Article
- Essentiality of c-di-AMP in Bacillus subtilis: Bypassing mutations converge in potassium and glutamate homeostasis.PLoS genetics · 2021Article
- The energy landscape of -1 ribosomal frameshifting.Science advances · 2020Article
- Active role of elongation factor G in maintaining the mRNA reading frame during translation.Science advances · 2019Article
- Spontaneous ribosomal translocation of mRNA and tRNAs into a chimeric hybrid state.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- New Insights into Ribosome Structure and Function.Cold Spring Harbor perspectives in biology · 2019Review
- How Messenger RNA and Nascent Chain Sequences Regulate Translation Elongation.Annual review of biochemistry · 2018Review
- Eukaryotic translation elongation factor 2 (eEF2) catalyzes reverse translocation of the eukaryotic ribosome.The Journal of biological chemistry · 2018Article
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During translation, elongation factor G (EF-G) catalyzes the translocation of tRNA2-mRNA inside the ribosome. Translocation is coupled to a cycle of conformational rearrangements of the ribosomal machinery, and how EF-G initiates translocation remains unresolved. Here we performed systematic mutagenesis of Escherichia coli EF-G and analyzed inhibitory single-site mutants of EF-G that preserved pretranslocation (Pre)-state ribosomes with tRNAs in A/P and P/E sites (Pre-EF-G). Our results suggest that the interactions between the decoding center and the codon-anticodon duplex constitute the barrier for translocation. Catalysis of translocation by EF-G involves the factor's highly conserved loops I and II at the tip of domain IV, which disrupt the hydrogen bonds between the decoding center and the duplex to release the latter, hence inducing subsequent translocation events, namely 30S head swiveling and tRNA2-mRNA movement on the 30S subunit.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.