ArticleMetabolic engineering2014
Toward aldehyde and alkane production by removing aldehyde reductase activity in Escherichia coli.
Article in Metabolic engineering, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 143 citations in OpenAlex.
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- Expression and characterization of monofunctional alcohol dehydrogenase enzymes inMetabolic engineering communications · 2024Article
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- Production of Fatty Acids and Derivatives Using Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- Utilizing Alcohol for Alkane Biosynthesis by Introducing a Fatty Alcohol Dehydrogenase.Applied and environmental microbiology · 2022Article
- High-throughput colorimetric assays optimized for detection of ketones and aldehydes produced by microbial cell factories.Microbial biotechnology · 2022Article
- Toward bioproduction of oxo chemicals from CBiotechnology for biofuels and bioproducts · 2022Review
- α-Dioxygenases (α-DOXs): Promising Biocatalysts for the Environmentally Friendly Production of Aroma Compounds.Chembiochem : a European journal of chemical biology · 2022Review
- Selective biosynthesis of retinol in S. cerevisiae.Bioresources and bioprocessing · 2022Article
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- Overexpression of Oxidoreductase YghA Confers Tolerance of Furfural in Ethanologenic Escherichia coli Strain SSK42.Applied and environmental microbiology · 2021Article
- An orthogonal metabolic framework for one-carbon utilization.Nature metabolism · 2021Article
- Production of l-Theanine by Escherichia coli in the Absence of Supplemental Ethylamine.Applied and environmental microbiology · 2021Article
- Production of Aldehydes by Biocatalysis.International journal of molecular sciences · 2021Review
- Aldehyde Production in Crude Lysate- and Whole Cell-Based Biotransformation Using a Noncanonical Redox Cofactor System.ACS catalysis · 2020Article
- Engineering a nicotinamide mononucleotide redox cofactor system for biocatalysis.Nature chemical biology · 2020Article
- Continuous photoproduction of hydrocarbon drop-in fuel by microbial cell factories.Scientific reports · 2019Article
- Article
- On the use of oxygenic photosynthesis for the sustainable production of commodity chemicals.Physiologia plantarum · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Advances in synthetic biology and metabolic engineering have enabled the construction of novel biological routes to valuable chemicals using suitable microbial hosts. Aldehydes serve as chemical feedstocks in the synthesis of rubbers, plastics, and other larger molecules. Microbial production of alkanes is dependent on the formation of a fatty aldehyde intermediate which is converted to an alkane by an aldehyde deformylating oxygenase (ADO). However, microbial hosts such as Escherichia coli are plagued by many highly active endogenous aldehyde reductases (ALRs) that convert aldehydes to alcohols, which greatly complicates strain engineering for aldehyde and alkane production. It has been shown that the endogenous ALR activity outcompetes the ADO enzyme for fatty aldehyde substrate. The large degree of ALR redundancy coupled with an incomplete database of ALRs represents a significant obstacle in engineering E. coli for either aldehyde or alkane production. In this study, we identified 44 ALR candidates encoded in the E. coli genome using bioinformatics tools, and undertook a comprehensive screening by measuring the ability of these enzymes to produce isobutanol. From the pool of 44 candidates, we found five new ALRs using this screening method (YahK, DkgA, GldA, YbbO, and YghA). Combined deletions of all 13 known ALRs resulted in a 90-99% reduction in endogenous ALR activity for a wide range of aldehyde substrates (C2-C12). Elucidation of the ALRs found in E. coli could guide one in reducing competing alcohol formation during alkane or aldehyde production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.