ArticleApplied and environmental microbiology2014
Axenic culture of a candidate division TM7 bacterium from the human oral cavity and biofilm interactions with other oral bacteria.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 84 citations in OpenAlex.
- Trial
- Using Genomics to Decipher the Enigmatic Properties and Survival Adaptation of Candidate Phyla Radiation.Microorganisms · 2023Review
- Advances in the oral microbiota and rapid detection of oral infectious diseases.Frontiers in microbiology · 2023Review
- Article
- Candidate Phyla Radiation, an Underappreciated Division of the Human Microbiome, and Its Impact on Health and Disease.Clinical microbiology reviews · 2022Review
- Insight into the Relationship between Oral Microbiota and the Inflammatory Bowel Disease.Microorganisms · 2022Review
- Polymicrobial Interactions of Oral Microbiota: a Historical Review and Current Perspective.mBio · 2022Review
- New Beta-lactamases in Candidate Phyla Radiation: Owning Pleiotropic Enzymes Is a Smart Paradigm for Microorganisms with a Reduced Genome.International journal of molecular sciences · 2022Article
- Reverse Genomics: Design of Universal Epitope Sets to Isolate AllMicroorganisms · 2022Article
- [Microbial Diversity and Community Analysis of Dental Plaques in Orthodontic Patients Wearing Invisible Appliances and Fixed Appliances].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2022Article
- Metabolic Potential of the Superphylum Patescibacteria Reconstructed from Activated Sludge Samples from a Municipal Wastewater Treatment Plant.Microbes and environments · 2022Article
- Evaluation of biofilm formation on different clear orthodontic retainer materials.Journal of orthodontic science · 2022Article
- New frontiers of oral sciences: Focus on the source and biomedical application of extracellular vesicles.Frontiers in bioengineering and biotechnology · 2022Review
- Dataset for Genome Sequencing and De Novo Assembly of the Candidate Phyla Radiation in Supragingival Plaque.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2022Article
- Adapted Protocol forMicrobiology spectrum · 2021Article
- Microbial diversity in tropical marine sediments assessed using culture-dependent and culture-independent techniques.Environmental microbiology · 2021Article
- Article
- Cultivating the Bacterial Microbiota ofmSystems · 2021Article
- Rhizomal Reclassification of Living Organisms.International journal of molecular sciences · 2021Review
- The composition of microbial communities in inflammatory periodontal diseases in young adults Tatars.AIMS microbiology · 2021Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The diversity of bacterial species in the human oral cavity is well recognized, but a high proportion of them are presently uncultivable. Candidate division TM7 bacteria are almost always detected in metagenomic studies but have not yet been cultivated. In this paper, we identified candidate division TM7 bacterial phylotypes in mature plaque samples from around orthodontic bonds in subjects undergoing orthodontic treatment. Successive rounds of enrichment in laboratory media led to the isolation of a pure culture of one of these candidate division TM7 phylotypes. The bacteria formed filaments of 20 to 200 μm in length within agar plate colonies and in monospecies biofilms on salivary pellicle and exhibited some unusual morphological characteristics by transmission electron microscopy, including a trilaminated cell surface layer and dense cytoplasmic deposits. Proteomic analyses of cell wall protein extracts identified abundant polypeptides predicted from the TM7 partial genomic sequence. Pleiomorphic phenotypes were observed when the candidate division TM7 bacterium was grown in dual-species biofilms with representatives of six different oral bacterial genera. The TM7 bacterium formed long filaments in dual-species biofilm communities with Actinomyces oris or Fusobacterium nucleatum. However, the TM7 isolate grew as short rods or cocci in dual-species biofilms with Porphyromonas gingivalis, Prevotella intermedia, Parvimonas micra, or Streptococcus gordonii, forming notably robust biofilms with the latter two species. The ability to cultivate TM7 axenically should majorly advance understanding of the physiology, genetics, and virulence properties of this novel candidate division oral bacterium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.