ReviewPlasmid2014
Mechanisms of plasmid segregation: have multicopy plasmids been overlooked?
Review in Plasmid, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 102 citations in OpenAlex.
- Spatial constraints determine the spread of plasmid-encoded antibiotic resistance between bacterial colonies.mSystems · 2026Article
- The shared evolutionary capacities of plasmids and extrachromosomal DNA.Nature reviews. Genetics · 2026Review
- Plasmids inClinical microbiology reviews · 2026Review
- Mobile Genetic Elements as Central Drivers of Antimicrobial Resistance: Molecular Mechanisms, Evolutionary Ecology, One Health Implications and Control Strategies.Antibiotics (Basel, Switzerland) · 2026Review
- Beyond bactericidal: targeting plasmid-mediated antibiotic resistance with natural product-based plasmid curing agents.Frontiers in microbiology · 2026Review
- A noncanonical intrinsic terminator in the HicAB toxin-antitoxin operon promotes the transmission of conjugative antibiotic resistance plasmids.Nucleic acids research · 2025Article
- Leveraging EngineeredACS synthetic biology · 2025Article
- Episomal virus maintenance enables bacterial population recovery from infection and promotes virus-bacterial coexistence.The ISME journal · 2025Article
- Antibiotic use in oyster hatcheries promotes rapid spread of a highly transferable and modular resistance plasmid in Vibrio.The ISME journal · 2025Article
- No two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum.Microbial cell factories · 2024Article
- A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli.Microbial cell factories · 2024Article
- Application of plasmid stabilization systems for heterologous protein expression in Escherichia coli.Molecular biology reports · 2024Article
- Bacteria can maintain rRNA operons solely on plasmids for hundreds of millions of years.Nature communications · 2023Article
- The parABSm system is involved in megaplasmid partitioning and genome integrity maintenance in Thermus thermophilus.G3 (Bethesda, Md.) · 2023Article
- Molecular Analysis of pSK1 par: A Novel Plasmid Partitioning System Encoded by Staphylococcal Multiresistance Plasmids.Journal of molecular biology · 2022Article
- Fixation dynamics of beneficial alleles in prokaryotic polyploid chromosomes and plasmids.Genetics · 2022Article
- Confinement anisotropy drives polar organization of two DNA molecules interacting in a nanoscale cavity.Nature communications · 2022Article
- Modeling endonuclease colicin-like bacteriocin operons as 'genetic arms' in plasmid-genome conflicts.Molecular genetics and genomics : MGG · 2022Article
- Construction of an Antibiotic-Free Vector and its Application in the Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2022Article
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Plasmids are self-replicating pieces of DNA typically bearing non-essential genes. Given that plasmids represent a metabolic burden to the host, mechanisms ensuring plasmid transmission to daughter cells are critical for their stable maintenance in the population. Here we review these mechanisms, focusing on two active partition strategies common to low-copy plasmids: par systems type I and type II. Both involve three components: an adaptor protein, a motor protein, and a centromere, which is a sequence area in the plasmid that is recognized by the adaptor protein. The centromere-bound adaptor nucleates polymerization of the motor, leading to filament formation, which can pull plasmids apart (par I) or push them towards opposite poles of the cell (par II). No such active partition mechanisms are known to occur in high copy number plasmids. In this case, vertical transmission is generally considered stochastic, due to the random distribution of plasmids in the cytoplasm. We discuss conceptual and experimental lines of evidence questioning the random distribution model and posit the existence of a mechanism for segregation in high copy number plasmids that moves plasmids to cell poles to facilitate transmission to daughter cells. This mechanism would involve chromosomally-encoded proteins and the plasmid origin of replication. Modulation of this proposed mechanism of segregation could provide new ways to enhance plasmid stability in the context of recombinant gene expression, which is limiting for large-scale protein production and for bioremediation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.