ArticleNucleic acids research2014
Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD.Nature structural & molecular biology · 2023Article
- Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance.The EMBO journal · 2023Article
- Nucleic Acid Probes for Single-Molecule Localization Imaging of Cellular Biomolecules.Chemical & biomedical imaging · 2023Review
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
- Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- GDSC SMLM: Single-molecule localisation microscopy software for ImageJ.Wellcome open research · 2022Article
- Bound2Learn: a machine learning approach for classification of DNA-bound proteins from single-molecule tracking experiments.Nucleic acids research · 2021Article
- Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo.eLife · 2021Article
- Fluorescent Protein-Based Indicators for Functional Super-Resolution Imaging of Biomolecular Activities in Living Cells.International journal of molecular sciences · 2019Review
- The PCNA unloader Elg1 promotes recombination at collapsed replication forks in fission yeast.eLife · 2019Article
- Citrullination of HP1γ chromodomain affects association with chromatin.Epigenetics & chromatin · 2019Article
- FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells.Nature communications · 2018Article
- Activation of the Notch Signaling Pathway In Vivo Elicits Changes in CSL Nuclear Dynamics.Developmental cell · 2018Article
- Recruitment of UvrBC complexes to UV-induced damage in the absence of UvrA increases cell survival.Nucleic acids research · 2018Article
- Evaluating single molecule detection methods for microarrays with high dynamic range for quantitative single cell analysis.Scientific reports · 2017Article
- Combining Primed Photoconversion and UV-Photoactivation for Aberration-Free, Live-Cell Compliant Multi-Color Single-Molecule Localization Microscopy Imaging.International journal of molecular sciences · 2017Article
- PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.PLoS genetics · 2017Article
- PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells.Scientific reports · 2016Article
- Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells.Methods in molecular biology (Clifton, N.J.) · 2016Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms. Application of these methodologies to understanding processes underlying DNA replication and repair has been limited to defined in vitro biochemical analysis and prokaryotic cells. In order to expand these techniques to eukaryotic systems, we have further developed a photo-activated localization microscopy-based method to directly visualize DNA-associated proteins in unfixed eukaryotic cells. We demonstrate that motion blurring of fluorescence due to protein diffusivity can be used to selectively image the DNA-bound population of proteins. We designed and tested a simple methodology and show that it can be used to detect changes in DNA binding of a replicative helicase subunit, Mcm4, and the replication sliding clamp, PCNA, between different stages of the cell cycle and between distinct genetic backgrounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.