ArticleMolecular & cellular proteomics : MCP2014
Ion mobility tandem mass spectrometry enhances performance of bottom-up proteomics.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed.
- Bimodal Peptide Collision Cross Section Distribution Reflects Two Stable Conformations in the Gas Phase.Journal of proteome research · 2026Article
- Protein corona formed on lipid nanoparticles compromises delivery efficiency of mRNA cargo.Nature communications · 2025Article
- Gas Phase Separation of Modified Peptides for Activity-Based Protein Profiling.Analytical chemistry · 2025Article
- Proteomic Insights into Bacterial Responses to Antibiotics: A Narrative Review.International journal of molecular sciences · 2025Review
- Screening of Protein Carbonylation Sites in Human Serum by Ion Mobility Mass Spectrometry.Journal of proteome research · 2025Article
- Ion Mobility-Assisted Free Radical-Initiated Peptide Sequencing.International journal of mass spectrometry · 2025Article
- ProPept-MT: A Multi-Task Learning Model for Peptide Feature Prediction.International journal of molecular sciences · 2024Article
- Instrumentation at the Leading Edge of Proteomics.Analytical chemistry · 2024Review
- The One Hour Human Proteome.Molecular & cellular proteomics : MCP · 2024Article
- Peptide collision cross sections of 22 post-translational modifications.Analytical and bioanalytical chemistry · 2023Article
- Orbitrap-Based Mass and Charge Analysis of Single Molecules.Accounts of chemical research · 2023Article
- Structure of mycobacterial respiratory complex I.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Antibiotic Resistance Diagnosis in ESKAPE Pathogens-A Review on Proteomic Perspective.Diagnostics (Basel, Switzerland) · 2023Review
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- Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.Chemical reviews · 2022Review
- A comprehensive LFQ benchmark dataset on modern day acquisition strategies in proteomics.Scientific data · 2022Article
- Evaluation of Disposable Trap Column nanoLC-FAIMS-MS/MS for the Proteomic Analysis of FFPE Tissue.Journal of proteome research · 2021Article
- Analysis of the Endogenous Peptidomes of Different Infant Formula Types and Human Milk.Foods (Basel, Switzerland) · 2021Article
- Quantitative LC-MS/MS uncovers the regulatory role of autophagy in immune thrombocytopenia.Cancer cell international · 2021Article
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17 authors.
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No grant is acknowledged in the PubMed record.
Abstract
One of the limiting factors in determining the sensitivity of tandem mass spectrometry using hybrid quadrupole orthogonal acceleration time-of-flight instruments is the duty cycle of the orthogonal ion injection system. As a consequence, only a fraction of the generated fragment ion beam is collected by the time-of-flight analyzer. Here we describe a method utilizing postfragmentation ion mobility spectrometry of peptide fragment ions in conjunction with mobility time synchronized orthogonal ion injection leading to a substantially improved duty cycle and a concomitant improvement in sensitivity of up to 10-fold for bottom-up proteomic experiments. This enabled the identification of 7500 human proteins within 1 day and 8600 phosphorylation sites within 5 h of LC-MS/MS time. The method also proved powerful for multiplexed quantification experiments using tandem mass tags exemplified by the chemoproteomic interaction analysis of histone deacetylases with Trichostatin A.
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