Evidence map›Paper›PMID 25104411›Full record

ArticleJournal of biomedical optics2014

Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy.

Chun-Rui Hu, Delong Zhang, Mikhail N Slipchenko, Ji-Xin Cheng, Bing Hu

Open access · bronzeAbstract read
In one paragraph

Article in Journal of biomedical optics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Brain & spine · 2022
    Review
  6. Review
  7. Advances in stimulated Raman scattering imaging for tissues and animals.Quantitative imaging in medicine and surgery · 2021
    Review
  8. Article
  9. Article
  10. Article
  11. Imaging MyelinationBrain plasticity (Amsterdam, Netherlands) · 2016
    Review
  12. Article
  13. Coherent Raman Scattering Microscopy in Biology and Medicine.Annual review of biomedical engineering · 2015
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Chun-Rui HuUniversity of Science and Technology of China, Hefei National Laboratory for Physical Sciences at Microscale, and the School of Life Sciences, Hefei 230027, China.
Delong ZhangPurdue University, Department of Chemistry, West Lafayette, Indiana 47907, United States.
Mikhail N SlipchenkoPurdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, United States.
Ji-Xin ChengPurdue University, Department of Chemistry, West Lafayette, Indiana 47907, United StatescPurdue University, Weldon School of Biomedical Engineering, West Lafayette, Indiana 47907, United States.
Bing HuUniversity of Science and Technology of China, Hefei National Laboratory for Physical Sciences at Microscale, and the School of Life Sciences, Hefei 230027, China.
Hefei National Center for Physical Sciences at Nanoscale · CNPurdue University West Lafayette · US

Funding

Label-free multichannel spectral cytometry based on stimulated Raman scatteringR21GM104681 · NIGMS · PURDUE UNIVERSITY · PI CHENG, JI-XIN · 2012 to 2014
$571k
NIBIB NIH HHS R21EB105901NIGMS NIH HHS R21 GM104681NIGMS NIH HHS R21GM104681
6 · The paper itself

Abstract

The myelin sheath plays an important role as the axon in the functioning of the neural system, and myelin degradation is a hallmark pathology of multiple sclerosis and spinal cord injury. Electron microscopy, fluorescent microscopy, and magnetic resonance imaging are three major techniques used for myelin visualization. However, microscopic observation of myelin in living organisms remains a challenge. Using a newly developed stimulated Raman scattering microscopy approach, we report noninvasive, label-free, real-time in vivo imaging of myelination by a single-Schwann cell, maturation of a single node of Ranvier, and myelin degradation in the transparent body of the Xenopus laevis tadpole.

Indexed as

AnimalsEquipment DesignEquipment Failure AnalysisImage EnhancementMicroscopyMyelin SheathNerve Fibers, MyelinatedReproducibility of ResultsSensitivity and SpecificitySpectrum Analysis, RamanXenopus laevis

Identifiers

PMID25104411
PMCPMC4407663
OpenAlexW2142012703

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.