ArticleJournal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry2014
Comparative investigation of the reaction mechanisms of the organophosphate-degrading phosphotriesterases from Agrobacterium radiobacter (OpdA) and Pseudomonas diminuta (OPH).
Article in Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 59 citations in OpenAlex.
- Distribution and Evolutionary Signatures of a Broad-Spectrum Pesticide-Degrading Carboxylesterase TriS From Strain S113.Environmental microbiology · 2026Article
- Adsorptive Removal Behavior of Two Activated Carbons for Bis(2-ethylhexyl) Phosphate Dissolved in Water.Toxics · 2025Article
- Structural and Functional Insight into the Mechanism of the Fe-S Cluster-Dependent Dehydratase from Paralcaligenes ureilyticus.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Overview of a bioremediation tool: organophosphorus hydrolase and its significant application in the food, environmental, and therapy fields.Applied microbiology and biotechnology · 2021Review
- Rapid, Biomimetic Degradation of a Nerve Agent Simulant by Incorporating Imidazole Bases into a Metal-Organic Framework.ACS catalysis · 2021Article
- Environmental Occurrence, Toxicity Concerns, and Degradation of Diazinon Using a Microbial System.Frontiers in microbiology · 2021Review
- Easy-to-use and reliable absorbance-based MPH-GST biosensor for the detection of methyl parathion pesticide.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Structure and mechanism of potent bifunctional β-lactam- and homoserine lactone-degrading enzymes from marine microorganisms.Scientific reports · 2020Article
- Rapid Biodegradation of the Organophosphorus Insecticide Chlorpyrifos byInternational journal of environmental research and public health · 2019Article
- Aminoalcohol-Induced Activation of Organophosphorus Hydrolase (OPH) towards Diisopropylfluorophosphate (DFP).PloS one · 2017Article
- Product release is rate-limiting for catalytic processing by the Dengue virus protease.Scientific reports · 2016Article
- Use of magnetic circular dichroism to study dinuclear metallohydrolases and the corresponding biomimetics.European biophysics journal : EBJ · 2015Review
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Authors and funding
9 authors at 5 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Metal ion-dependent, organophosphate-degrading enzymes have acquired increasing attention due to their ability to degrade and thus detoxify commonly used pesticides and nerve agents such as sarin. The best characterized of these enzymes are from Pseudomonas diminuta (OPH) and Agrobacterium radiobacter (OpdA). Despite high sequence homology (>90 % identity) and conserved metal ion coordination these enzymes display considerable variations in substrate specificity, metal ion affinity/preference and reaction mechanism. In this study, we highlight the significance of the presence (OpdA) or absence (OPH) of an extended hydrogen bond network in the active site of these enzymes for the modulation of their catalytic properties. In particular, the second coordination sphere residue in position 254 (Arg in OpdA, His in OPH) is identified as a crucial factor in modulating the substrate preference and binding of these enzymes. Inhibition studies with fluoride also support a mechanism for OpdA whereby the identity of the hydrolysis-initiating nucleophile changes as the pH is altered. The same is not observed for OPH.
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