ArticleNucleic acids research2014
Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 108 citations in OpenAlex.
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- The Glycine-Rich RNA-Binding Protein Is a Vital Post-Transcriptional Regulator in Crops.Plants (Basel, Switzerland) · 2023Review
- microRNA biogenesis and stabilization in plants.Fundamental research · 2023Review
- Cotranscriptional RNA processing and modification in plants.The Plant cell · 2023Review
- Extracellular RNA: mechanisms of secretion and potential functions.Journal of experimental botany · 2023Article
- Functional role of microRNA in the regulation of biotic and abiotic stress in agronomic plants.Frontiers in genetics · 2023Review
- microRNA production inFrontiers in plant science · 2023Review
- The MYB33, MYB65, and MYB101 transcription factors affect Arabidopsis and potato responses to drought by regulating the ABA signaling pathway.Physiologia plantarum · 2022Article
- Identification of Pri-miRNA Stem-Loop Interacting Proteins in Plants Using a Modified Version of the Csy4 CRISPR Endonuclease.International journal of molecular sciences · 2022Article
- Du13 encodes a CPlant biotechnology journal · 2022Article
- Arabidopsis apoplastic fluid contains sRNA- and circular RNA-protein complexes that are located outside extracellular vesicles.The Plant cell · 2022Article
- Mechanisms of MicroRNA Biogenesis and Stability Control in Plants.Frontiers in plant science · 2022Review
- Recent advances in the regulation of plant miRNA biogenesis.RNA biology · 2021Review
- Searching for G-Quadruplex-Binding Proteins in Plants: New Insight into Possible G-Quadruplex Regulation.Biotech (Basel (Switzerland)) · 2021Article
- Article
- Regulation of pri-MIRNA processing: mechanistic insights into the miRNA homeostasis in plant.Plant cell reports · 2021Review
- Improved Functional Expression of Cytochrome P450s inFrontiers in bioengineering and biotechnology · 2021Article
- Pollen-Specific Protein PSP231 Activates Callose Synthesis to Govern Male Gametogenesis and Pollen Germination.Plant physiology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hnRNP-like glycine-rich RNA-binding protein AtGRP7 regulates pre-mRNA splicing in Arabidopsis. Here we used small RNA-seq to show that AtGRP7 also affects the miRNA inventory. AtGRP7 overexpression caused a significant reduction in the level of 30 miRNAs and an increase for 14 miRNAs with a minimum log2 fold change of ± 0.5. Overaccumulation of several pri-miRNAs including pri-miR398b, pri-miR398c, pri-miR172b, pri-miR159a and pri-miR390 at the expense of the mature miRNAs suggested that AtGRP7 affects pri-miRNA processing. Indeed, RNA immunoprecipitation revealed that AtGRP7 interacts with these pri-miRNAs in vivo. Mutation of an arginine in the RNA recognition motif abrogated in vivo binding and the effect on miRNA and pri-miRNA levels, indicating that AtGRP7 inhibits processing of these pri-miRNAs by direct binding. In contrast, pri-miRNAs of selected miRNAs that were elevated or not changed in response to high AtGRP7 levels were not bound in vivo. Reduced accumulation of miR390, an initiator of trans-acting small interfering RNA (ta-siRNA) formation, also led to lower TAS3 ta-siRNA levels and increased mRNA expression of the target AUXIN RESPONSE FACTOR4. Furthermore, AtGRP7 affected splicing of pri-miR172b and pri-miR162a. Thus, AtGRP7 is an hnRNP-like protein with a role in processing of pri-miRNAs in addition to its role in pre-mRNA splicing.
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