ArticleThe New phytologist2014
Identification and functional assay of the interaction motifs in the partner protein OsNAR2.1 of the two-component system for high-affinity nitrate transport.
Article in The New phytologist, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 73 citations in OpenAlex.
- SaNAR2.2, A Novel Member of the NAR2 Family from the EuhalophyteMembranes · 2026Article
- A genetic module boosts grain yield and nitrogen use efficiency by improving nitrate transport in maize.Nature genetics · 2026Article
- Unveiling novel QTLs for nitrogen use efficiency in temperate Japonica rice.BMC plant biology · 2025Article
- Spinach SoNRT3 Interacts with SoNRT2a to Improve Low-Nitrogen Tolerance via Nitrate Uptake and Root Growth.Plants (Basel, Switzerland) · 2025Article
- 9311 allele of OsNAR2.2 enhances nitrate transport to improve rice yield and nitrogen use efficiency.Plant biotechnology journal · 2025Article
- BdNRT2A and BdNRT3.2 Are the Major Components of the High-Affinity Nitrate Transport System inPlant direct · 2025Article
- Adaptive dynamics of extrachromosomal circular DNA in rice under nutrient stress.Nature communications · 2025Article
- OsNLP3 and OsPHR2 orchestrate direct and mycorrhizal pathways for nitrate uptake by regulating NAR2.1-NRT2s complexes in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Physiological, molecular, and environmental insights into plant nitrogen uptake, and metabolism under abiotic stresses.The plant genome · 2024Review
- Overexpression of OsNAR2.1 by OsNAR2.1 promoter increases drought resistance by increasing the expression of OsPLDα1 in rice.BMC plant biology · 2024Article
- Nuclear translocation of OsMADS25 facilitated by OsNAR2.1 in reponse to nitrate signals promotes rice root growth by targeting OsMADS27 and OsARF7.Plant communications · 2023Article
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- Functional characterization of thePeerJ · 2023Article
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- Nitrate signaling and use efficiency in crops.Plant communications · 2022Review
- PlantGSAD: a comprehensive gene set annotation database for plant species.Nucleic acids research · 2022Article
- Understanding plant-microbe interaction of rice and soybean with two contrasting diazotrophic bacteria through comparative transcriptome analysis.Frontiers in plant science · 2022Article
- Molecular Regulatory Networks for Improving Nitrogen Use Efficiency in Rice.International journal of molecular sciences · 2021Review
- Proofing Direct-Seeded Rice with Better Root Plasticity and Architecture.International journal of molecular sciences · 2021Review
- Plant DNA methylation is sensitive to parent seed N content and influences the growth of rice.BMC plant biology · 2021Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
A partner protein, NAR2, is essential for high-affinity nitrate transport of the NRT2 protein in plants. However, the NAR2 motifs that interact with NRT2s for their plasma membrane (PM) localization and nitrate transporter activity have not been functionally characterized. In this study, OsNAR2.1 mutations with different carbon (C)-terminal deletions and nine different point mutations in the conserved regions of NAR2 homologs in plants were generated to explore the essential motifs involved in the interaction with OsNRT2.3a. Screening using the membrane yeast two-hybrid system and Xenopus oocytes for nitrogen-15 ((15)N) uptake demonstrated that either R100G or D109N point mutations impaired the OsNAR2.1 interaction with OsNRT2.3a. Western blotting and visualization using green fluorescent protein fused to either the N- or C-terminus of OsNAR2.1 indicated that OsNAR2.1 is expressed in both the PM and cytoplasm. The split-yellow fluorescent protein (YFP)/BiFC analyses indicated that OsNRT2.3a was targeted to the PM in the presence of OsNAR2.1, while either R100G or D109N mutation resulted in the loss of OsNRT2.3a-YFP signal in the PM. Based on these results, arginine 100 and aspartic acid 109 of the OsNAR2.1 protein are key amino acids in the interaction with OsNRT2.3a, and their interaction occurs in the PM but not cytoplasm.
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