ArticleVirology journal2014
Validation of an automated ELISA system for detection of antibodies to Aleutian mink disease virus using blood samples collected in filter paper strips.
Article in Virology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 29 citations in OpenAlex.
- A novel high-sensitivity TaqMan qPCR assay reveals amdoparvovirus DNA in zoo-housed small mammals in southern China.Veterinary research communications · 2026Article
- Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison).PloS one · 2024Article
- A developed TaqMan probe-based qPCR was used to quantify the distribution of AMDV in various tissues of infected mink and its prevalence in northern China.Frontiers in veterinary science · 2024Article
- Review
- Serum Analytes of American Mink (Neovison Vison) Challenged with Aleutian Mink Disease Virus.Animals : an open access journal from MDPI · 2022Article
- Long-term antibody production and viremia in American mink (Neovison vison) challenged with Aleutian mink disease virus.BMC veterinary research · 2022Article
- Applying Machine Learning Algorithms for the Classification of Mink Infected with Aleutian Disease Using Different Data Sources.Animals : an open access journal from MDPI · 2022Article
- Dried Blood Spots technology for veterinary applications and biological investigations: technical aspects, retrospective analysis, ongoing status and future perspectives.Veterinary research communications · 2022Review
- Seroprevalence and Molecular Epidemiology of Aleutian Disease in Various Countries during 1972-2021: A Review and Meta-Analysis.Animals : an open access journal from MDPI · 2021Review
- AMDV Vaccine: Challenges and Perspectives.Viruses · 2021Review
- Dose response of black American mink to Aleutian mink disease virus.Immunity, inflammation and disease · 2020Article
- Evaluation of methods for detection of asymptomatic individuals infected with Leishmania infantum in the state of Piauí, Brazil.PLoS neglected tropical diseases · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAleutian mink disease virus (AMDV) is the cause of a chronic immune complex disease, Aleutian disease (AD), which is common in mink-producing countries. In 2005, implementation of an AMDV eradication programme in Finland created a need for an automated high-throughput assay. The aim of this study was to validate an AMDV-VP2 -recombinant antigen ELISA, which we developed earlier, in an automated assay format for the detection of anti-AMDV antibodies in mink blood and to determine the accuracy of this test compared with the reference standard (counter-current immunoelectrophoresis, CIEP).
methodsA blood sampling method based on filter paper 12-strips (blood combs) and a device to introduce these strips to an ELISA plate for elution of the samples were developed. Blood and serum samples were collected from 761 mink from two farms with low (2%) and high (81%) seroprevalences of AMDV infection in 2008. ELISA sensitivity and specificity were estimated with a Bayesian 2-test 2-population model that allowed for conditional dependence between CIEP and ELISA. Agreement between the two tests was assessed with kappa statistic and proportion agreement.
resultsThe sensitivity and specificity of the automated ELISA system were estimated to be 96.2% and 98.4%, respectively. Agreement between CIEP and ELISA was high, with a kappa value of 0.976 and overall proportion agreement of 98.8%.
conclusionsThe automated ELISA system combined with blood comb sampling is an accurate test format for the detection of anti-AMDV antibodies in mink blood and offers several advantages, including improved blood sampling and data handling, fast sample throughput time, and reductions in costs and labour inputs.
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