ArticleMolecular biology of the cell2014
The Caenorhabditis elegans pericentriolar material components SPD-2 and SPD-5 are monomeric in the cytoplasm before incorporation into the PCM matrix.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Protein phase separation: new insights into cell division.Acta biochimica et biophysica Sinica · 2023Article
- A modified TurboID approach identifies tissue-specific centriolar components in C. elegans.PLoS genetics · 2022Article
- Human centrosome organization and function in interphase and mitosis.Seminars in cell & developmental biology · 2021Review
- Sulforaphane Targets TRA-1/GLI Upstream of DAF-16/FOXO to PromoteFrontiers in cell and developmental biology · 2021Article
- A two-step mechanism for the inactivation of microtubule organizing center function at the centrosome.eLife · 2019Article
- Mitotic Cell Division inGenetics · 2019Review
- Bridging centrioles and PCM in proper space and time.Essays in biochemistry · 2018Review
- Functions and dysfunctions of the mammalian centrosome in health, disorders, disease, and aging.Histochemistry and cell biology · 2018Review
- Phosphatase PP2A and microtubule-mediated pulling forces disassemble centrosomes during mitotic exit.Biology open · 2018Article
- Polo-like kinase phosphorylation determines Caenorhabditis elegans centrosome size and density by biasing SPD-5 toward an assembly-competent conformation.Biology open · 2016Article
- ATX-2, the C. elegans Ortholog of Human Ataxin-2, Regulates Centrosome Size and Microtubule Dynamics.PLoS genetics · 2016Article
- A centrosome interactome provides insight into organelle assembly and reveals a non-duplication role for Plk4.Nature communications · 2016Article
- MLL5 maintains spindle bipolarity by preventing aberrant cytosolic aggregation of PLK1.The Journal of cell biology · 2016Article
- Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP.eLife · 2015Article
- Centrosome function and assembly in animal cells.Nature reviews. Molecular cell biology · 2015Review
- Isotropic incorporation of SPD-5 underlies centrosome assembly in C. elegans.Current biology : CB · 2015Article
- Building a ninefold symmetrical barrel: structural dissections of centriole assembly.Open biology · 2015Review
- Centrosomes. Regulated assembly of a supramolecular centrosome scaffold in vitro.Science (New York, N.Y.) · 2015Article
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10 authors.
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Abstract
Centrosomes are the main microtubule-organizing centers in animal cells. Centrosomes consist of a pair of centrioles surrounded by a matrix of pericentriolar material (PCM) that assembles from cytoplasmic components. In Caenorhabditis elegans embryos, interactions between the coiled-coil proteins SPD-5 and SPD-2 and the kinase PLK-1 are critical for PCM assembly. However, it is not known whether these interactions promote the formation of cytoplasmic complexes that are added to the PCM or whether the components interact only during incorporation into the PCM matrix. Here we address this problem by using a combination of live-cell fluorescence correlation spectroscopy, mass spectrometry, and hydrodynamic techniques to investigate the native state of PCM components in the cytoplasm. We show that SPD-2 is monomeric, and neither SPD-2 nor SPD-5 exists in complex with PLK-1. SPD-5 exists mostly as a monomer but also forms complexes with the PP2A-regulatory proteins RSA-1 and RSA-2, which are required for microtubule organization at centrosomes. These results suggest that the interactions between SPD-2, SPD-5, and PLK-1 do not result in formation of cytoplasmic complexes, but instead occur in the context of PCM assembly.
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