ArticleJournal of virological methods2014
Development of an algorithm for production of inactivated arbovirus antigens in cell culture.
Article in Journal of virological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Extracellular Expression and Diagnostic Potential of Dengue Virus Type 3 E Protein Domain III Through Codon Optimization in Komagataella phaffii.Molecular biotechnology · 2026Article
- Development and evaluation of recombinant E2 protein based IgM capture enzyme-linked immunosorbent assay (ELISA) and double antigen sandwich ELISA for detection of antibodies to Chikungunya virus.PLoS neglected tropical diseases · 2022Article
- Epidemiology of Shuni Virus in Horses in South Africa.Viruses · 2021Article
- Comparison of Zika virus inactivation methods for reagent production and disinfection methods.Journal of virological methods · 2021Article
- Chikungunya E2 Protein Produced inViruses · 2020Article
- Automated application of low energy electron irradiation enables inactivation of pathogen- and cell-containing liquids in biomedical research and production facilities.Scientific reports · 2020Article
- Generation of a Lineage II Powassan Virus (Deer Tick Virus) cDNA Clone: Assessment of Flaviviral Genetic Determinants of Tick and Mosquito Vector Competence.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2018Article
- Evaluation of the inactivation of Venezuelan equine encephalitis virus by several common methods.Journal of virological methods · 2018Article
- Biotechnological Applications of an Insect-Specific Alphavirus.DNA and cell biology · 2017Article
- Full inactivation of alphaviruses in single particle and crystallized forms.Journal of virological methods · 2016Article
- Development and validation of an ELISA kit (YF MAC-HD) to detect IgM to yellow fever virus.Journal of virological methods · 2015Article
- Production of a Sindbis/Eastern Equine Encephalitis chimeric virus inactivated cell culture antigen.Journal of virological methods · 2015Article
- Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection.PLoS neglected tropical diseases · 2015Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Arboviruses are medically important pathogens that cause human disease ranging from a mild fever to encephalitis. Laboratory diagnosis is essential to differentiate arbovirus infections from other pathogens with similar clinical manifestations. The Arboviral Diseases Branch (ADB) reference laboratory at the CDC Division of Vector-Borne Diseases (DVBD) produces reference antigens used in serological assays such as the virus-specific immunoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA). Antigen production in cell culture has largely replaced the use of suckling mice; however, the methods are not directly transferable. The development of a cell culture antigen production algorithm for nine arboviruses from the three main arbovirus families, Flaviviridae, Togaviridae, and Bunyaviridae, is described here. Virus cell culture growth and harvest conditions were optimized, inactivation methods were evaluated, and concentration procedures were compared for each virus. Antigen performance was evaluated by the MAC-ELISA at each step of the procedure. The antigen production algorithm is a framework for standardization of methodology and quality control; however, a single antigen production protocol was not applicable to all arboviruses and needed to be optimized for each virus.
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Registered trials
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