ArticleDevelopmental biology2014
Polarized exocyst-mediated vesicle fusion directs intracellular lumenogenesis within the C. elegans excretory cell.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 60 citations in OpenAlex.
- Integrin is required for basement membrane crossing and branching of an invading intracellular tube.Development (Cambridge, England) · 2026Article
- The dynamics of tubulogenesis in development and disease.Development (Cambridge, England) · 2025Review
- Exocyst stimulates multiple steps of exocytic SNARE complex assembly and vesicle fusion.Nature structural & molecular biology · 2025Article
- MRCK-1 activates non-muscle myosin for outgrowth of a unicellular tube in Caenorhabditis elegans.Development (Cambridge, England) · 2024Article
- Branched-chain actin dynamics polarizes vesicle trajectories and partitions apicobasal epithelial membrane domains.Science advances · 2023Article
- The biosynthetic-secretory pathway, supplemented by recycling routes, determines epithelial membrane polarity.Science advances · 2023Article
- A signalling cascade for Ral.Small GTPases · 2022Review
- Parallel Rap1>RalGEF>Ral and Ras signals sculpt the C. elegans nervous system.Developmental biology · 2021Article
- Article
- Epithelial morphogenesis, tubulogenesis and forces in organogenesis.Current topics in developmental biology · 2021Review
- Terminal web and vesicle trafficking proteins mediate nematode single-cell tubulogenesis.The Journal of cell biology · 2020Article
- Mutations in the exocyst component EXOC2 cause severe defects in human brain development.The Journal of experimental medicine · 2020Article
- A Series of Tubes: TheJournal of developmental biology · 2020Review
- The Role ofGenetics · 2020Article
- The postmitotic midbody: Regulating polarity, stemness, and proliferation.The Journal of cell biology · 2019Review
- A tensile trilayered cytoskeletal endotube drives capillary-like lumenogenesis.The Journal of cell biology · 2019Article
- NovelG3 (Bethesda, Md.) · 2019Article
- Developmental fidelity is imposed by genetically separable RalGEF activities that mediate opposing signals.PLoS genetics · 2019Article
- Tubular Excretory Canal Structure Depends on Intermediate Filaments EXC-2 and IFA-4 inGenetics · 2018Article
- 14-3-3εa directs the pulsatile transport of basal factors toward the apical domain for lumen growth in tubulogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Lumenogenesis of small seamless tubes occurs through intracellular membrane growth and directed vesicle fusion events. Within the Caenorhabditis elegans excretory cell, which forms seamless intracellular tubes (canals) that mediate osmoregulation, lumens grow in length and diameter when vesicles fuse with the expanding lumenal surface. Here, we show that lumenal vesicle fusion depends on the small GTPase RAL-1, which localizes to vesicles and acts through the exocyst vesicle-tethering complex. Loss of either the exocyst or RAL-1 prevents excretory canal lumen extension. Within the excretory canal and other polarized cells, the exocyst co-localizes with the PAR polarity proteins PAR-3, PAR-6 and PKC-3. Using early embryonic cells to determine the functional relationships between the exocyst and PAR proteins, we show that RAL-1 recruits the exocyst to the membrane, while PAR proteins concentrate membrane-localized exocyst proteins to a polarized domain. These findings reveal that RAL-1 and the exocyst direct the polarized vesicle fusion events required for intracellular lumenogenesis of the excretory cell, suggesting mechanistic similarities in the formation of topologically distinct multicellular and intracellular lumens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.