ArticlePLoS genetics2014
A system for genome-wide histone variant dynamics in ES cells reveals dynamic MacroH2A2 replacement at promoters.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Multifunctional histone variants in genome function.Nature reviews. Genetics · 2025Review
- Article
- macroH2A2 antagonizes epigenetic programs of stemness in glioblastoma.Nature communications · 2023Article
- Rtt109 promotes nucleosome replacement ahead of the replication fork.Genome research · 2022Article
- The histone variant macroH2A1.1 regulates RNA polymerase II-paused genes within defined chromatin interaction landscapes.Journal of cell science · 2022Article
- Chromatin structure-dependent histone incorporation revealed by a genome-wide deposition assay.eLife · 2021Article
- LSH mediates gene repression through macroH2A deposition.Nature communications · 2020Article
- An embryonic stem cell-specific heterochromatin state promotes core histone exchange in the absence of DNA accessibility.Nature communications · 2020Article
- Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells.Epigenetics · 2020Article
- Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains.The Journal of cell biology · 2019Article
- A hyperdynamic H3.3 nucleosome marks promoter regions in pluripotent embryonic stem cells.Nucleic acids research · 2017Article
- Software for rapid time dependent ChIP-sequencing analysis (TDCA).BMC bioinformatics · 2017Article
- Understanding nucleosome dynamics and their links to gene expression and DNA replication.Nature reviews. Molecular cell biology · 2017Review
- The histone variant macroH2A confers functional robustness to the intestinal stem cell compartment.PloS one · 2017Article
- Loss of histone variant macroH2A2 expression associates with progression of anal neoplasm.Journal of clinical pathology · 2016Article
- Article
- Article
- Histone H3.3 is required for endogenous retroviral element silencing in embryonic stem cells.Nature · 2015Article
- Histone variants as emerging regulators of embryonic stem cell identity.Epigenetics · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Dynamic exchange of a subset of nucleosomes in vivo plays important roles in epigenetic inheritance of chromatin states, chromatin insulator function, chromosome folding, and the maintenance of the pluripotent state of embryonic stem cells. Here, we extend a pulse-chase strategy for carrying out genome-wide measurements of histone dynamics to several histone variants in murine embryonic stem cells and somatic tissues, recapitulating expected characteristics of the well characterized H3.3 histone variant. We extended this system to the less-studied MacroH2A2 variant, commonly described as a "repressive" histone variant whose accumulation in chromatin is thought to fix the epigenetic state of differentiated cells. Unexpectedly, we found that while large intergenic blocks of MacroH2A2 were stably associated with the genome, promoter-associated peaks of MacroH2A2 exhibited relatively rapid exchange dynamics in ES cells, particularly at highly-transcribed genes. Upon differentiation to embryonic fibroblasts, MacroH2A2 was gained primarily in additional long, stably associated blocks across gene-poor regions, while overall turnover at promoters was greatly dampened. Our results reveal unanticipated dynamic behavior of the MacroH2A2 variant in pluripotent cells, and provide a resource for future studies of tissue-specific histone dynamics in vivo.
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