ArticlePLoS pathogens2014
Cyclic di-GMP-dependent signaling pathways in the pathogenic Firmicute Listeria monocytogenes.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 101 citations in OpenAlex.
- The conserved Pss exopolysaccharide is a common component ofJournal of bacteriology · 2026Article
- Network-level divergence in cyclic di-GMP signalling drives ecological versatility in Acinetobacter baumannii.NPJ biofilms and microbiomes · 2026Article
- Induction of the viable but non-culturable state by an alkyl dipropylene triamine-based disinfectant in Vibrio parahaemolyticus biofilms.Scientific reports · 2025Article
- Disinfectant-induced bacterial resistance and antibiotic cross-resistance-mechanisms and clinical relevance.Clinical and experimental medicine · 2025Review
- Plasmid-free cheater cells commonly evolve during laboratory growth.Applied and environmental microbiology · 2024Article
- Maple compounds prevent biofilm formation inFrontiers in microbiology · 2024Article
- Large Multicountry Outbreak of Invasive Listeriosis by a Listeria monocytogenes ST394 Clone Linked to Smoked Rainbow Trout, 2020 to 2021.Microbiology spectrum · 2023Article
- Bactericidal Action of Shrimp Shell Chitooligosaccharide Conjugated with Epigallocatechin Gallate (COS-EGCG) againstFoods (Basel, Switzerland) · 2023Article
- Biofilm Signaling, Composition and Regulation inJournal of microbiology and biotechnology · 2023Review
- TheFrontiers in microbiology · 2023Article
- Lignan-containing maple products inhibitFrontiers in microbiology · 2023Article
- A comprehensive investigation of protein expression profiles inFrontiers in microbiology · 2023Article
- Benzalkonium chloride disinfectant residues stimulate biofilm formation and increase survival ofFrontiers in microbiology · 2023Article
- Impact of benzalkonium chloride, benzethonium chloride and chloroxylenol on bacterial antimicrobial resistance.Journal of applied microbiology · 2022Review
- Extracellular c-di-GMP Plays a Role in Biofilm Formation and Dispersion ofMicroorganisms · 2022Article
- The Involvement of Thiamine Uptake in the Virulence ofPathogens (Basel, Switzerland) · 2022Article
- cAMP and c-di-GMP synergistically support biofilm maintenance through the direct interaction of their effectors.Nature communications · 2022Article
- Broadcasting of amplitude- and frequency-modulated c-di-GMP signals facilitates cooperative surface commitment in bacterial lineages.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- NrnA Is a Linear Dinucleotide Phosphodiesterase with Limited Function in Cyclic Dinucleotide Metabolism in Listeria monocytogenes.Journal of bacteriology · 2022Article
- TheMicroorganisms · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
We characterized key components and major targets of the c-di-GMP signaling pathways in the foodborne pathogen Listeria monocytogenes, identified a new c-di-GMP-inducible exopolysaccharide responsible for motility inhibition, cell aggregation, and enhanced tolerance to disinfectants and desiccation, and provided first insights into the role of c-di-GMP signaling in listerial virulence. Genome-wide genetic and biochemical analyses of c-di-GMP signaling pathways revealed that L. monocytogenes has three GGDEF domain proteins, DgcA (Lmo1911), DgcB (Lmo1912) and DgcC (Lmo2174), that possess diguanylate cyclase activity, and three EAL domain proteins, PdeB (Lmo0131), PdeC (Lmo1914) and PdeD (Lmo0111), that possess c-di-GMP phosphodiesterase activity. Deletion of all phosphodiesterase genes (ΔpdeB/C/D) or expression of a heterologous diguanylate cyclase stimulated production of a previously unknown exopolysaccharide. The synthesis of this exopolysaccharide was attributed to the pssA-E (lmo0527-0531) gene cluster. The last gene of the cluster encodes the fourth listerial GGDEF domain protein, PssE, that functions as an I-site c-di-GMP receptor essential for exopolysaccharide synthesis. The c-di-GMP-inducible exopolysaccharide causes cell aggregation in minimal medium and impairs bacterial migration in semi-solid agar, however, it does not promote biofilm formation on abiotic surfaces. The exopolysaccharide also greatly enhances bacterial tolerance to commonly used disinfectants as well as desiccation, which may contribute to survival of L. monocytogenes on contaminated food products and in food-processing facilities. The exopolysaccharide and another, as yet unknown c-di-GMP-dependent target, drastically decrease listerial invasiveness in enterocytes in vitro, and lower pathogen load in the liver and gallbladder of mice infected via an oral route, which suggests that elevated c-di-GMP levels play an overall negative role in listerial virulence.
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