ArticleColombia medica (Cali, Colombia)
Comparison of seven diagnostic tests to detect Trypanosoma cruzi infection in patients in chronic phase of Chagas disease.
Article in Colombia medica (Cali, Colombia). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.
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Who cites it
21 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Molecular diagnosis of Chagas disease: a systematic review and meta-analysis.Infectious diseases of poverty · 2023Pooled it
- Rapid immunochromatographic tests for the diagnosis of chronic Chagas disease in at-risk populations: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2019Pooled it
- Diagnostic methods for protozoan diseases: a review focused on leishmaniasis, Chagas disease and malaria.Frontiers in microbiology · 2026Review
- The use of peptides for immunodiagnosis of human Chagas disease.Amino acids · 2024Review
- Article
- Laboratory diagnosis ofFrontiers in parasitology · 2023Review
- Accuracy of Diagnostic Tests for the Detection of Chagas Disease: A Systematic Review and Meta-Analysis.Diagnostics (Basel, Switzerland) · 2022Review
- Evaluation of the Performance of Ortho T. cruzi ELISA Test System for the Detection of Antibodies to Trypanosoma cruzi.Journal of clinical microbiology · 2022Article
- Mapping the morbidity and mortality of Chagas disease in an endemic area in Brazil.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease.Pathogens (Basel, Switzerland) · 2021Article
- Diversity and Epidemiology of Bat Trypanosomes: A One Health Perspective.Pathogens (Basel, Switzerland) · 2021Review
- Evaluation of UMELISA CHAGAS™ with the incorporation of new monomeric and chimeric peptides representative of different regions of Trypanosoma cruziBiomedica : revista del Instituto Nacional de Salud · 2021Article
- Performance Evaluation of a Commercial Real-Time PCR Assay and of an In-House Real-Time PCR forMicroorganisms · 2020Article
- Chagas Disease: Detection ofJournal of clinical medicine · 2020Article
- Evaluation of the analytical and diagnostic performance of a digital droplet polymerase chain reaction (ddPCR) assay to detect Trypanosoma cruzi DNA in blood samples.PLoS neglected tropical diseases · 2018Article
- Biosensors to Diagnose Chagas Disease: A Brief Review.Sensors (Basel, Switzerland) · 2017Review
- Molecular Diagnosis of Chagas Disease in Colombia: Parasitic Loads and Discrete Typing Units in Patients from Acute and Chronic Phases.PLoS neglected tropical diseases · 2016Article
- Chronic Chagas Disease Diagnosis: A Comparative Performance of Commercial Enzyme Immunoassay Tests.The American journal of tropical medicine and hygiene · 2016Article
- Preventing the transmission of American trypanosomiasis and its spread into non-endemic countries.Infectious diseases of poverty · 2015Review
- Highly discordant serology against Trypanosoma cruzi in central Veracruz, Mexico: role of the antigen used for diagnostic.Parasites & vectors · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo compare the diagnostic performance of seven methods to determine Trypanosoma cruzi infection in patients with chronic Chagas disease.
methodsAnalytical study, using the case-control design, which included 205 people (patients with Chagasic cardiomyopathy, n=100; control group, n=105). Three enzyme linked immunosorbent assays, one indirect hemagglutination assay and one immunochromatographic test were assessed. Additionally, DNA amplification was performed via the PCR method using kinetoplast and nuclear DNA as target sequences. For the comparative analysis of diagnostic tests, the parameters used were sensitivity, specificity, positive and negative predictive values, Receiver Operator Characteristic (ROC), positive and negative likelihood ratio, as well as κ quality analysis.
resultsThe commercial Bioelisa Chagas test showed the highest sensitivity (98%), specificity (100%), and positive and negative predictive values; additionally, it had the highest discriminatory power. Otherwise, the amplification of T. cruzi DNA in blood samples showed low values of sensitivity (kinetoplast DNA = 51%, nuclear DNA = 22%), but high values of specificity (100%), and moderate to low discriminatory ability.
conclusionThe comparative analysis among the different methods suggests that the diagnostic strategy of T. cruzi infection in patients with chronic Chagas disease can be performed using ELISA assays based on recombinant proteins and/or synthetic peptides, which show higher diagnosis performance and can confirm and exclude the diagnosis of T. cruzi infection. The molecular methods show poor performance when used in the diagnosis of patients with chronic Chagas disease.
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Registered trials
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