ArticleMolecular & cellular proteomics : MCP2014
Quantitative profiling of chromatome dynamics reveals a novel role for HP1BP3 in hypoxia-induced oncogenesis.
Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Biomarkers Identification in the Microenvironment of Oral Squamous Cell Carcinoma: A Systematic Review of Proteomic Studies.International journal of molecular sciences · 2024Pooled it
- Native chromatome profiling reveals hundreds of metabolic enzymes in the nucleus across tissues.Nature communications · 2026Article
- Single-cell atlas of transcriptomic vulnerability across multiple neurodegenerative and neuropsychiatric diseases.medRxiv : the preprint server for health sciences · 2024Article
- Identification and partial characterization of new cell density-dependent nucleocytoplasmic shuttling proteins and open chromatin.Scientific reports · 2023Article
- Investigating phase separation properties of chromatin-associated proteins using gradient elution of 1,6-hexanediol.BMC genomics · 2023Article
- Flavylium-Based Hypoxia-Responsive Probe for Cancer Cell Imaging.Molecules (Basel, Switzerland) · 2021Article
- Promoter G-quadruplex folding precedes transcription and is controlled by chromatin.Genome biology · 2021Article
- Application of Advanced Mass Spectrometry-Based Proteomics to Study Hypoxia Driven Cancer Progression.Frontiers in oncology · 2021Review
- Identifying Susceptibility Loci for Cutaneous Squamous Cell Carcinoma Using a Fast Sequence Kernel Association Test.Frontiers in genetics · 2021Article
- HP1BP3 promotes tumor growth and metastasis by upregulating miR-23a to target TRAF5 in esophageal squamous cell carcinoma.American journal of cancer research · 2021Article
- Article
- Gene transcription and chromatin regulation in hypoxia.Biochemical Society transactions · 2020Review
- Proteome dynamics analysis identifies functional roles of SDE2 and hypoxia in DNA damage response in prostate cancer cells.NAR cancer · 2020Article
- ERO1α promotes hypoxic tumor progression and is associated with poor prognosis in pancreatic cancer.Oncotarget · 2019Article
- Pulsed SILAC-based proteomic analysis unveils hypoxia- and serum starvation-inducedOncotarget · 2019Article
- Hypoxia and Chromatin: A Focus on Transcriptional Repression Mechanisms.Biomedicines · 2018Review
- The novel transcriptional factor HP1BP3 negatively regulates Hsp70 transcription in Crassostrea hongkongensis.Scientific reports · 2017Article
- Article
- The differential expression of alternatively polyadenylated transcripts is a common stress-induced response mechanism that modulates mammalian mRNA expression in a quantitative and qualitative fashion.RNA (New York, N.Y.) · 2016Article
- Changes in gene expression of histone modification enzymes in rat granulosa cells undergoing luteinization during ovulation.Journal of ovarian research · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to the intensely studied genetic and epigenetic changes that induce host cell transformation to initiate tumor development, those that promote the malignant progression of cancer remain poorly defined. As emerging evidence suggests that the hypoxic tumor microenvironment could re-model the chromatin-associated proteome (chromatome) to induce epigenetic changes and alter gene expression in cancer cells, we hypothesized that hypoxia-driven evolution of the chromatome promotes malignant changes and the development of therapy resistance in tumor cells. To test this hypothesis, we isolated chromatins from tumor cells treated with varying conditions of normoxia, hypoxia, and re-oxygenation and then partially digested them with DNase I and analyzed them for changes in euchromatin- and heterochromatin-associated proteins using an iTRAQ-based quantitative proteomic approach. We identified a total of 1446 proteins with a high level of confidence, including 819 proteins that were observed to change their chromatin association topology under hypoxic conditions. These hypoxia-sensitive proteins included key mediators of chromatin organization, transcriptional regulation, and DNA repair. Furthermore, our proteomic and functional experiments revealed a novel role for the chromatin organizer protein HP1BP3 in mediating chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance, and self-renewal. Taken together, our findings indicate that HP1BP3 is a key mediator of tumor progression and cancer cell acquisition of therapy-resistant traits, and thus might represent a novel therapeutic target in a range of human malignancies.
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