ArticleBiology of reproduction2014
Acrosome reaction and Ca²⁺ imaging in single human spermatozoa: new regulatory roles of [Ca²⁺]i.
Article in Biology of reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 74 citations in OpenAlex.
- Molecular interplay between sperm and oocyte: a narrative review.Human reproduction update · 2026Review
- The Fer Tyrosine Kinase Mediates EGFR Activation in Sperm Capacitation.International journal of molecular sciences · 2025Article
- Real-Time Acrosome Reaction and Intracellular Calcium Recordings in Mice Sperm.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Real-Time Imaging of Calcium Dynamics in Human Sperm After Precise Single-Cell Stimulation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization.eLife · 2024Article
- Ultraviolet Filters: Dissecting Current Facts and Myths.Journal of clinical medicine · 2024Review
- Review
- Mechanisms That Protect Mammalian Sperm from the Spontaneous Acrosome Reaction.International journal of molecular sciences · 2023Review
- SKF96365 modulates activity of CatSper channels in human sperm.Molecular human reproduction · 2023Article
- α-Synuclein is required for sperm exocytosis at a post-fusion stage.Frontiers in cell and developmental biology · 2023Article
- Recombinant human OVGP1 increases intracellular calcium and further potentiates the effects of progesterone on human sperm.Journal of assisted reproduction and genetics · 2022Article
- Elevated and Sustained Intracellular Calcium Signalling Is Necessary for Efficacious Induction of the Human Sperm Acrosome Reaction.International journal of molecular sciences · 2022Article
- Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution.International journal of molecular sciences · 2021Article
- Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- The Action of Reproductive Fluids and Contained Steroids, Prostaglandins, and ZnFrontiers in cell and developmental biology · 2021Article
- Discrete Dynamic Model of the Mammalian Sperm Acrosome Reaction: The Influence of Acrosomal pH and Physiological Heterogeneity.Frontiers in physiology · 2021Article
- Mathematical model reveals that heterogeneity in the number of ion transporters regulates the fraction of mouse sperm capacitation.PloS one · 2021Article
- Behavioural switching during oscillations of intracellular CaReproduction & fertility · 2021Article
- Utilization of bull fertility-associated antigen to improve the quality of frozen bull semen.Veterinary world · 2020Article
- Release of Porcine Sperm from Oviduct Cells is Stimulated by Progesterone and Requires CatSper.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The spermatozoa acrosome reaction (AR) is essential for mammalian fertilization. Few methods allow visualization of AR in real time together with Ca²⁺ imaging. Here, we show that FM4-64, a fluorescent dye used to follow exocytosis, reliably reports AR progression induced by ionomycin and progesterone in human spermatozoa. FM4-64 clearly delimits the spermatozoa contour and reports morphological cell changes before, during, and after AR. This strategy unveiled the formation of moving tubular appendages, emerging from acrosome-reacted spermatozoa, which was confirmed by scanning electron microscopy. Alternate wavelength illumination allowed concomitant imaging of FM4-64 and Fluo-4, a Ca²⁺ indicator. These AR and intracellular Ca²⁺ ([Ca²⁺]i) recordings revealed that the presence of [Ca²⁺]i oscillations, both spontaneous and progesterone induced, prevents AR in human spermatozoa. Notably, the progesterone-induced AR is preceded by a second [Ca²⁺]i peak and ~40% of reacting spermatozoa also manifest a slow [Ca²⁺]i rise ~2 min before AR. Our findings uncover new AR features related to [Ca²⁺]i.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.