ArticleDevelopment (Cambridge, England)2014
Deadenylase depletion protects inherited mRNAs in primordial germ cells.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Article
- Live imaging of echinoderm embryos to illuminate evo-devo.Frontiers in cell and developmental biology · 2022Article
- Micromere formation and its evolutionary implications in the sea urchin.Current topics in developmental biology · 2022Article
- A single cell RNA sequencing resource for early sea urchin development.Development (Cambridge, England) · 2020Article
- Evolutionary modification of AGS protein contributes to formation of micromeres in sea urchins.Nature communications · 2019Article
- Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.Developmental biology · 2019Article
- Maternal Nanos inhibits Importin-α2/Pendulin-dependent nuclear import to prevent somatic gene expression in the Drosophila germline.PLoS genetics · 2019Article
- Identifying gene expression from single cells to single genes.Methods in cell biology · 2019Review
- Genome-wide analysis of chromatin accessibility using ATAC-seq.Methods in cell biology · 2019Review
- Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs).Methods in cell biology · 2019Review
- From genome to anatomy: The architecture and evolution of the skeletogenic gene regulatory network of sea urchins and other echinoderms.Genesis (New York, N.Y. : 2000) · 2018Review
- Nodal induces sequential restriction of germ cell factors during primordial germ cell specification.Development (Cambridge, England) · 2018Article
- Article
- A quiet space during rush hour: Quiescence in primordial germ cells.Stem cell research · 2017Article
- Article
- Report from the 2016 University of California, San Francisco, Center for Reproductive Sciences retreat.Molecular reproduction and development · 2017Article
- Transient translational quiescence in primordial germ cells.Development (Cambridge, England) · 2017Article
- Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin.Developmental biology · 2016Article
- Primordial germ cells: the first cell lineage or the last cells standing?Development (Cambridge, England) · 2015Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
A crucial event in animal development is the specification of primordial germ cells (PGCs), which become the stem cells that create sperm and eggs. How PGCs are created provides a valuable paradigm for understanding stem cells in general. We find that the PGCs of the sea urchin Strongylocentrotus purpuratus exhibit broad transcriptional repression, yet enrichment for a set of inherited mRNAs. Enrichment of several germline determinants in the PGCs requires the RNA-binding protein Nanos to target the transcript that encodes CNOT6, a deadenylase, for degradation in the PGCs, thereby creating a stable environment for RNA. Misexpression of CNOT6 in the PGCs results in their failure to retain Seawi transcripts and Vasa protein. Conversely, broad knockdown of CNOT6 expands the domain of Seawi RNA as well as exogenous reporters. Thus, Nanos-dependent spatially restricted CNOT6 differential expression is used to selectively localize germline RNAs to the PGCs. Our findings support a 'time capsule' model of germline determination, whereby the PGCs are insulated from differentiation by retaining the molecular characteristics of the totipotent egg and early embryo.
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