ArticleOpen biology2014
Insights into DNA hydroxymethylation in the honeybee from in-depth analyses of TET dioxygenase.
Article in Open biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 70 citations in OpenAlex.
- DNA methylation dynamics associated with seasonal wing plasticity of Bicyclus anynana butterflies.Molecular biology and evolution · 2026Article
- Global research trends on the relationship between RNA mBiophysics reports · 2026Article
- Integration of epigenetics into ecotoxicology: insights and fundamental research needs.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Genome-wide demethylation and targeted remethylation during metamorphosis in the jewel wasp Nasonia vitripennis.Epigenetics & chromatin · 2025Article
- RNA modifications in cancer.MedComm · 2025Review
- Is the concept of mammalian epigenetic clocks universal and applicable to invertebrates?Frontiers in genetics · 2025Article
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- Active DNA demethylation of developmentalScience advances · 2022Article
- Biological roles of RNA mBiomarker research · 2022Review
- Review
- DNA Methylation in Honey Bees and the Unresolved Questions in Insect Methylomics.Advances in experimental medicine and biology · 2022Article
- DNA methylation across the tree of life, from micro to macro-organism.Bioengineered · 2022Article
- Article
- Phage-encoded ten-eleven translocation dioxygenase (TET) is active in C5-cytosine hypermodification in DNA.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Novel structure in the nuclei of honey bee brain neurons revealed by immunostaining.Scientific reports · 2021Article
- Reversal of nucleobase methylation by dioxygenases.Nature chemical biology · 2020Review
- Epigenetic Molecular Mechanisms in Insects.Neotropical entomology · 2020Review
- Exploring DNA Methylation Diversity in the Honey Bee Brain by Ultra-Deep Amplicon Sequencing.Epigenomes · 2020Article
- DNA Methylation and Demethylation Are Regulated by Functional DNA Methyltransferases and DnTET Enzymes inFrontiers in genetics · 2020Article
- Characterization of a Dopamine Transporter and Its Splice Variant Reveals Novel Features of Dopaminergic Regulation in the Honey Bee.Frontiers in physiology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammals, a family of TET enzymes producing oxidized forms of 5-methylcytosine (5mC) plays an important role in modulating DNA demethylation dynamics. In contrast, nothing is known about the function of a single TET orthologue present in invertebrates. Here, we show that the honeybee TET (AmTET) catalytic domain has dioxygenase activity and converts 5mC to 5-hydroxymethylcytosine (5hmC) in a HEK293T cell assay. In vivo, the levels of 5hmC are condition-dependent and relatively low, but in testes and ovaries 5hmC is present at approximately 7-10% of the total level of 5mC, which is comparable to that reported for certain mammalian cells types. AmTET is alternatively spliced and highly expressed throughout development and in adult tissues with the highest expression found in adult brains. Our findings reveal an additional level of flexible genomic modifications in the honeybee that may be important for the selection of multiple pathways controlling contrasting phenotypic outcomes in this species. In a broader context, our study extends the current, mammalian-centred attention to TET-driven DNA hydroxymethylation to an easily manageable organism with attractive and unique biology.
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