ArticleNature communications2014
Single-molecule force spectroscopy reveals force-enhanced binding of calcium ions by gelsolin.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Mechanochemistry: Fundamental Principles and Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- A Hierarchical Mechanotransduction System: From Macro to Micro.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- The role of single protein elasticity in mechanobiology.Nature reviews. Materials · 2023Article
- Protein folding mechanism revealed by single-molecule force spectroscopy experiments.Biophysics reports · 2021Article
- An ester bond underlies the mechanical strength of a pathogen surface protein.Nature communications · 2021Article
- Single-particle virology.Biophysical reviews · 2020Review
- Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes.Frontiers in molecular biosciences · 2020Review
- Multistep Protein Unfolding Scenarios from the Rupture of a Complex Metal Cluster CdScientific reports · 2019Article
- Functional characterization of alternatively spliced GSN in head and neck squamous cell carcinoma.Translational research : the journal of laboratory and clinical medicine · 2018Article
- Proteins Breaking Bad: A Free Energy Perspective.The journal of physical chemistry letters · 2017Article
- Multidomain proteins under force.Nanotechnology · 2017Article
- Unusually high mechanical stability of bacterial adhesin extender domains having calcium clamps.PloS one · 2017Article
- Kinetic Ductility and Force-Spike Resistance of Proteins from Single-Molecule Force Spectroscopy.Biophysical journal · 2016Article
- Mechano-adaptive sensory mechanism of α-catenin under tension.Scientific reports · 2016Article
- Adaptable hydrogel networks with reversible linkages for tissue engineering.Advanced materials (Deerfield Beach, Fla.) · 2015Review
- High-resolution probing heparan sulfate-antithrombin interaction on a single endothelial cell surface: single-molecule AFM studies.Physical chemistry chemical physics : PCCP · 2015Article
- Force-induced remodelling of proteins and their complexes.Current opinion in structural biology · 2015Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Force is increasingly recognized as an important element in controlling biological processes. Forces can deform native protein conformations leading to protein-specific effects. Protein-protein binding affinities may be decreased, or novel protein-protein interaction sites may be revealed, on mechanically stressing one or more components. Here we demonstrate that the calcium-binding affinity of the sixth domain of the actin-binding protein gelsolin (G6) can be enhanced by mechanical force. Our kinetic model suggests that the calcium-binding affinity of G6 increases exponentially with force, up to the point of G6 unfolding. This implies that gelsolin may be activated at lower calcium ion levels when subjected to tensile forces. The demonstration that cation-protein binding affinities can be force-dependent provides a new understanding of the complex behaviour of cation-regulated proteins in stressful cellular environments, such as those found in the cytoskeleton-rich leading edge and at cell adhesions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.