ArticleReproductive biology and endocrinology : RB&E2014
Androgenic regulation of beta-defensins in the mouse epididymis.
Article in Reproductive biology and endocrinology : RB&E, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
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- Single-cell transcriptomic and cross-species comparison analyses reveal distinct molecular changes of porcine testes during puberty.Communications biology · 2024Article
- Distinct actions of testicular endocrine and lumicrine signaling on the proximal epididymal transcriptome.Reproductive biology and endocrinology : RB&E · 2024Article
- Busulfan administration replicated the characteristics of the epididymal initial segment observed in mice lacking testis-epididymis lumicrine signaling.The Journal of reproduction and development · 2024Article
- A New GeneInternational journal of molecular sciences · 2023Article
- Defensins: defenders of human reproductive health.Human reproduction update · 2023Review
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- Differential gene expression and hallmarks of stemness in epithelial cells of the developing rat epididymis.Cell and tissue research · 2022Article
- The cecropin-prophenoloxidase regulatory mechanism is a cross-species physiological function in mosquitoes.iScience · 2022Article
- Declined expressing mRNA of beta-defensin 108 from epididymis is associated with decreased sperm motility in blue fox (Vulpes lagopus).BMC veterinary research · 2021Article
- Anatomical Transcriptome Atlas of the Male Mouse Reproductive System During Aging.Frontiers in cell and developmental biology · 2021Article
- Article
- Immunolocalization of androgen and vitamin D receptors in the epididymis of mature ram (Saudi journal of biological sciences · 2021Article
- Language Impairment with a Partial Duplication ofMolecular syndromology · 2020Article
- Seasonal expressions of SPAG11A and androgen receptor in the epididymis of the wild ground squirrels (<em>Citellus dauricus</em> Brandt).European journal of histochemistry : EJH · 2020Article
- Article
- Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ.Molecules (Basel, Switzerland) · 2016Article
- β-defensins and the epididymis: contrasting influences of prenatal, postnatal, and adult scenarios.Asian journal of andrologyReview
- Host-parasite Responses Outcome Regulate the Expression of Antimicrobial Peptide Genes in the Skin of BALB/c and C57BL/6 Murine Strains FollowingIranian journal of parasitologyArticle
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe majority of beta-defensin family members are exclusively expressed in the epididymis, and some members have been shown to play essential roles in sperm maturation and fertility in rats, mice and humans. Therefore, beta-defensins are hypothesized to be potential targets for contraception and infertility diagnosis and treatment. Clarifying the regulatory mechanisms for the expression of these genes is necessary. Androgen/androgen receptor (AR) signaling plays an important regulatory role in epididymal structure and function. However, very little is known about the androgenic regulation on the production and secretion of the epididymal beta-defensins.
methodsThe expression of beta-defensins was detected by quantitative RT-PCR. The androgen dependence of beta-defensins was determined by bilateral orchiectomy and androgen supplementation. The androgen response elements (AREs) in the promoters of beta-defensins were identified using the MatInspector software. The binding of AR to AREs was assayed by ChIP-PCR/qPCR.
resultsWe demonstrated that 23 mouse caput epididymal beta-defensins were differentially regulated by androgen/androgen receptor. Six genes, Defb18, 19, 20, 39, 41, and 42, showed full regulation by androgens. Ten genes, Defb15, 30, 34, 37, 40, 45, 51, 52, 22 and Spag11a, were partially regulated by androgens. Defb15, 18, 19, 20, 30, 34, 37, 39, 41, 42, 22 and Spag11a were associated with androgen receptor binding sites in their promoter or intronic regions, indicating direct regulation of AR. Six genes, Defb1, 12, 13, 29, 35, and spag11b/c, exhibited an androgen-independent expression pattern. One gene, Defb25, was highly dependent on testicular factors rather on androgens.
conclusionsThe present study provides novel insights into the mechanisms of androgen regulation on epididymal beta-defensins, enabling a better understanding of the function of beta-defensins in sperm maturation and fertility.
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