ArticleMolecular plant pathology2015
Identification and functional analysis of Penicillium digitatum genes putatively involved in virulence towards citrus fruit.
Article in Molecular plant pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 81 citations in OpenAlex.
- Green Mold of Citrus: Recent Insights intoJournal of agricultural and food chemistry · 2026Review
- An Overview of MajorJournal of fungi (Basel, Switzerland) · 2026Review
- Integrated genome and transcriptome analysis reveals pathogenic mechanisms of Calonectria eucalypti in Eucalyptus leaf blight.BMC genomics · 2025Article
- A conserved fungal effector disturbs CaNature communications · 2025Article
- Unravelling the interplay of nitrogen nutrition and the Botrytis cinerea pectin lyase BcPNL1 in modulating Arabidopsis thaliana susceptibility.Communications biology · 2025Article
- Wide transcriptional outlook to uncoverHeliyon · 2024Article
- Discovery and Transcriptional Profiling ofJournal of fungi (Basel, Switzerland) · 2024Article
- Transcriptomic investigation of the interaction between a biocontrol yeast, Papiliotrema terrestris strain PT22AV, and the postharvest fungal pathogen Penicillium expansum on apple.Communications biology · 2024Article
- Article
- In Silico Characterization of the Secretome of the Fungal PathogenJournal of fungi (Basel, Switzerland) · 2023Article
- The Role of ABA in the Interaction between Citrus Fruit andInternational journal of molecular sciences · 2022Article
- Antioxidant and Antitumor Activities of Newly Synthesized Hesperetin Derivatives.Molecules (Basel, Switzerland) · 2022Article
- Deciphering Molecular Determinants UnderlyingInternational journal of molecular sciences · 2022Article
- Unveiling the Role Displayed byJournal of fungi (Basel, Switzerland) · 2021Article
- Albedo- and Flavedo-Specific Transcriptome Profiling Related toFoods (Basel, Switzerland) · 2021Article
- Chitosan modulates Pochonia chlamydosporia gene expression during nematode egg parasitism.Environmental microbiology · 2021Article
- EFE-Mediated Ethylene Synthesis Is the Major Pathway in the Citrus Postharvest PathogenJournal of fungi (Basel, Switzerland) · 2020Article
- Citrus Postharvest Green Mold: Recent Advances in Fungal Pathogenicity and Fruit Resistance.Microorganisms · 2020Review
- Article
- Elucidation of the Initial Growth Process and the Infection Mechanism ofMicroorganisms · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fungus Penicillium digitatum, the causal agent of green mould rot, is the most destructive post-harvest pathogen of citrus fruit in Mediterranean regions. In order to identify P. digitatum genes up-regulated during the infection of oranges that may constitute putative virulence factors, we followed a polymerase chain reaction (PCR)-based suppression subtractive hybridization and cDNA macroarray hybridization approach. The origin of expressed sequence tags (ESTs) was determined by comparison against the available genome sequences of both organisms. Genes coding for fungal proteases and plant cell wall-degrading enzymes represent the largest categories in the subtracted cDNA library. Northern blot analysis of a selection of P. digitatum genes, including those coding for proteases, cell wall-related enzymes, redox homoeostasis and detoxification processes, confirmed their up-regulation at varying time points during the infection process. Agrobacterium tumefaciens-mediated transformation was used to generate knockout mutants for two genes encoding a pectin lyase (Pnl1) and a naphthalene dioxygenase (Ndo1). Two independent P. digitatum Δndo1 mutants were as virulent as the wild-type. However, the two Δpnl1 mutants analysed were less virulent than the parental strain or an ectopic transformant. Together, these results provide a significant advance in our understanding of the putative determinants of the virulence mechanisms of P. digitatum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.