ArticleMolecular microbiology2014
Global analysis of mRNA decay intermediates in Bacillus subtilis wild-type and polynucleotide phosphorylase-deletion strains.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed.
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- Strategies for improving fengycin production: a review.Microbial cell factories · 2024Review
- Genome-wide annotation of transcript boundaries using bacterial Rend-seq datasets.Microbial genomics · 2024Article
- The previously uncharacterized RnpM (YlxR) protein modulates the activity of ribonuclease P in Bacillus subtilis in vitro.Nucleic acids research · 2024Article
- Bacillus subtilis NrnB is expressed during sporulation and acts as a unique 3'-5' exonuclease.Nucleic acids research · 2023Article
- Processing and decay of 6S-1 and 6S-2 RNAs inRNA (New York, N.Y.) · 2023Article
- Identification of Genes Required for Swarming Motility inJournal of bacteriology · 2022Article
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- Discovery and initial characterization of YloC, a novel endoribonuclease inRNA (New York, N.Y.) · 2022Article
- Polynucleotide phosphorylase and RNA helicase CshA cooperate inRNA biology · 2021Article
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- Gene expression profile analysis and target gene discovery of Mycobacterium tuberculosis biofilm.Applied microbiology and biotechnology · 2021Article
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- Bacterial ribonucleases and their roles in RNA metabolism.Critical reviews in biochemistry and molecular biology · 2019Review
- PNPase is involved in the coordination of mRNA degradation and expression in stationary phase cells of Escherichia coli.BMC genomics · 2018Article
- Maturation of polycistronic mRNAs by the endoribonuclease RNase Y and its associated Y-complex inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- RNases and Helicases in Gram-Positive Bacteria.Microbiology spectrum · 2018Article
- Genome-wide analysis shows that RNase G plays a global role in the stability of mRNAs in Stenotrophomonas maltophilia.Scientific reports · 2017Article
- Global survey of mRNA levels and decay rates ofHeliyon · 2017Article
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Abstract
Messenger RNA decay in Bacillus subtilis is accomplished by a combination of exoribonucleases and endoribonucleases. Intermediates in the decay process have not been readily detectable, and previous studies on mRNA decay have used a handful of highly expressed transcripts as models. Here, we use RNA-Seq analysis to probe mRNA turnover globally. A significant fraction of messages showed differential accumulation of RNA fragments that mapped near the 5' or 3' end of the coding sequence, consistent with initiation of decay from either the 5' end or from an internal cleavage site. Patterns of mRNA decay in the wild type were compared with patterns in a mutant strain lacking polynucleotide phosphorylase (PNPase), which is considered the major 3' exonuclease activity in mRNA decay and which is one of four known 3' exonucleases in B. subtilis. The results showed a striking dependence on PNPase for mRNA turnover in many cases, suggesting specificity in the ability of 3' exonucleases to degrade from 3'-hydroxyl termini. RNA-Seq data demonstrated a sharp decrease in expression of Sigma D in the PNPase-deletion strain. Reduction in sigD regulon expression explained the chain growth phenotype of the PNPase mutant and also predicted a defect in swarming motility.
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