ArticlePloS one2014
Sex determination in highly fragmented human DNA by high-resolution melting (HRM) analysis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- ONYX: an alignment-free biological sex inference from high-throughput sequencing data.Frontiers in bioinformatics · 2026Article
- Advancing non-destructive sex determination on human dental enamel using Raman spectroscopy.Scientific reports · 2025Article
- A qPCR-duplex assay for sex determination in ancient DNA.PloS one · 2022Article
- A novel method of male sex identification of human ancient skeletal remains.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- Review
- Alternatives to amelogenin markers for sex determination in humans and their forensic relevance.Molecular biology reports · 2020Review
- Genotyping genetic markers from LCN and degraded DNA by HRM and their application in hair shaft.International journal of legal medicine · 2020Article
- Evaluating macroscopic sex estimation methods using genetically sexed archaeological material: The medieval skeletal collection from St John's Divinity School, Cambridge.American journal of physical anthropology · 2019Article
- Liquid-to-gel transition for visual and tactile detection of biological analytes.Chemical communications (Cambridge, England) · 2017Article
- Sex estimation based on tooth measurements using panoramic radiographs.International journal of legal medicine · 2017Article
- Cooperation and tensions in multiethnic corporate societies using Teotihuacan, Central Mexico, as a case study.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Genetic Evidence Supports the Multiethnic Character of Teopancazco, a Neighborhood Center of Teotihuacan, Mexico (AD 200-600).PloS one · 2015Article
- Dental Evidence in Forensic Identification - An Overview, Methodology and Present Status.The open dentistry journal · 2015Article
- Forensic odontology as a humanitarian tool.Journal of oral and maxillofacial pathology : JOMFPReview
- Root Length: As a determinant tool of sexual dimorphism in an ethnic Tamil population.Journal of forensic dental sciencesArticle
- Sex determination from mesiodens of Indian children by amelogenin gene.Journal of forensic dental sciencesArticle
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex identification in ancient human remains is a common problem especially if the skeletons are sub-adult, incomplete or damaged. In this paper we propose a new method to identify sex, based on real-time PCR amplification of small fragments (61 and 64 bp) of the third exon within the amelogenin gene covering a 3-bp deletion on the AMELX-allele, followed by a High Resolution Melting analysis (HRM). HRM is based on the melting curves of amplified fragments. The amelogenin gene is located on both chromosomes X and Y, showing dimorphism in length. This molecular tool is rapid, sensitive and reduces the risk of contamination from exogenous genetic material when used for ancient DNA studies. The accuracy of the new method described here has been corroborated by using control samples of known sex and by contrasting our results with those obtained with other methods. Our method has proven to be useful even in heavily degraded samples, where other previously published methods failed. Stochastic problems such as the random allele drop-out phenomenon are expected to occur in a less severe form, due to the smaller fragment size to be amplified. Thus, their negative effect could be easier to overcome by a proper experimental design.
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