ArticlePloS one2014
Generation of Pet1210-Cre transgenic mouse line reveals non-serotonergic expression domains of Pet1 both in CNS and periphery.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Phenotypic Behavioural Effects of Genetic Deletion of the Vesicular Glutamate Transporter 3 in 5-Hydroxytryptamine Neurons.Genes, brain, and behavior · 2026Article
- Optogenetic Stimulation of Novel Tph2-Cre Rats Advances Insight into Serotonin's Role in Locomotion, Reinforcement, and Compulsivity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Impact of Serotonin Deficiency on Circadian Dopaminergic Rhythms.International journal of molecular sciences · 2024Article
- Evo-devo applied to sleep research: an approach whose time has come.Sleep advances : a journal of the Sleep Research Society · 2024Review
- D-aspartate oxidase gene duplication induces social recognition memory deficit in mice and intellectual disabilities in humans.Translational psychiatry · 2022Article
- Neurochemically and Hodologically Distinct Ascending VGLUT3 versus Serotonin Subsystems Comprise the r2-The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Article
- Separable gain control of ongoing and evoked activity in the visual cortex by serotonergic input.eLife · 2020Article
- Serotonergic Plasticity in the Dorsal Raphe Nucleus Characterizes Susceptibility and Resilience to Anhedonia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- The Stemness Gene Mex3A Is a Key Regulator of Neuroblast Proliferation During Neurogenesis.Frontiers in cell and developmental biology · 2020Article
- Characterization of transgenic mouse models targeting neuromodulatory systems reveals organizational principles of the dorsal raphe.Nature communications · 2019Article
- Embracing diversity in the 5-HT neuronal system.Nature reviews. Neuroscience · 2019Review
- Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord.Frontiers in cellular neuroscience · 2019Article
- Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord.Frontiers in molecular neuroscience · 2019Article
- Acute perturbation ofeLife · 2018Article
- Toward Serotonin Fluorescent False Neurotransmitters: Development of Fluorescent Dual Serotonin and Vesicular Monoamine Transporter Substrates for Visualizing Serotonin Neurons.ACS chemical neuroscience · 2018Article
- Serotonin neuron development: shaping molecular and structural identities.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Embryonic hindbrain patterning genes delineate distinct cardio-respiratory and metabolic homeostatic populations in the adult.Scientific reports · 2017Article
- Regulatory Mechanisms Controlling Maturation of Serotonin Neuron Identity and Function.Frontiers in cellular neuroscience · 2017Article
- Cellular architecture and transmitter phenotypes of neurons of the mouse median raphe region.Brain structure & function · 2017Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurons producing serotonin (5-hydroxytryptamine, 5-HT) constitute one of the most widely distributed neuronal networks in the mammalian central nervous system (CNS) and exhibit a profuse innervation throughout the CNS already at early stages of development. Serotonergic neuron specification is controlled by a combination of secreted molecules and transcription factors such as Shh, Fgf4/8, Nkx2.2, Lmx1b and Pet1. In the mouse, Pet1 mRNA expression appears between 10 and 11 days post coitum (dpc) in serotonergic post-mitotic precursors and persists in serotonergic neurons up to adulthood, where it promotes the expression of genes defining the mature serotonergic phenotype such as tryptophan hydroxylase 2 (Tph2) and serotonin transporter (SERT). Hence, the generation of genetic tools based on Pet1 specific expression represents a valuable approach to study the development and function of the serotonergic system. Here, we report the generation of a Pet1(210)-Cre transgenic mouse line in which the Cre recombinase is expressed under the control of a 210 kb fragment from the Pet1 genetic locus to ensure a reliable and faithful control of somatic recombination in Pet1 cell lineage. Besides Cre-mediated recombination accurately occurred in the serotonergic system as expected and according to previous studies, Pet1(210)-Cre transgenic mouse line allowed us to identify novel, so far uncharacterized, Pet1 expression domains. Indeed, we showed that in the raphe Pet1 is expressed also in a non-serotonergic neuronal population intermingled with Tph2-expressing cells and mostly localized in the B8 and B9 nuclei. Moreover, we detected Cre-mediated recombination also in the developing pancreas and in the ureteric bud derivatives of the kidney, where it reflected a specific Pet1 expression. Thus, Pet1(210)-Cre transgenic mouse line faithfully drives Cre-mediated recombination in all Pet1 expression domains representing a valuable tool to genetically manipulate serotonergic and non-serotonergic Pet1 cell lineages.
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