ArticleBlood2014
Hemolysis is a primary ATP-release mechanism in human erythrocytes.
Article in Blood, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 109 citations in OpenAlex.
- PlanHab Study: Consequences of combined normobaric hypoxia and bed rest on adenosine kinetics.Scientific reports · 2018Trial
- Effects of cranberry (Journal of the International Society of Sports Nutrition · 2017Trial
- Lysed erythrocyte membranes metabolically prime endothelial cells for angiogenesis.Angiogenesis · 2026Article
- Erythrocytes as Active Regulators of Blood Flow: A Brief Overview.Advances in experimental medicine and biology · 2026Review
- Pannexins in the vasculature.American journal of physiology. Heart and circulatory physiology · 2025Review
- Heme and iron toxicity in the aged spleen impairs T cell immunity through iron deprivation.Nature aging · 2025Article
- Pleiotropic Effects of Polymorphisms in theInternational journal of molecular sciences · 2025Article
- Role of shear stress-induced red blood cell released ATP in atherosclerosis.American journal of physiology. Heart and circulatory physiology · 2025Article
- Recombinant-Chemosynthetic Biosensors for Probing Cell Surface Signaling of Red Blood Cells and Other Cells.Chemical & biomedical imaging · 2025Article
- Effects of fermentedOpen veterinary journal · 2024Article
- Supplementing Glucose Intake Reverses the Inflammation Induced by a High-Fat Diet by Increasing the Expression of Siglec-E Ligands on Erythrocytes.Inflammation · 2024Article
- Alteration in the number, morphology, function, and metabolism of erythrocytes in high-altitude polycythemia.Frontiers in physiology · 2024Article
- Relationship between haematological data and radiation doses of TEPCO workers before and after the FDNNP accident.Journal of radiation research · 2023Article
- Heme oxygenase-1 as a predictor of sepsis-induced acute kidney injury: a cross-sectional study.Annals of translational medicine · 2022Article
- Possible role of pannexin 1 channels and purinergic receptors in the pathogenesis and mechanism of action of SARS-CoV-2 and therapeutic potential of targeting them in COVID-19.Life sciences · 2022Review
- Purinergic signaling is essential for full Psickle activation by hypoxia and by normoxic acid pH in mature human sickle red cells and in vitro-differentiated cultured human sickle reticulocytes.Pflugers Archiv : European journal of physiology · 2022Article
- The role of globins in cardiovascular physiology.Physiological reviews · 2022Review
- Fluorescence-coupled micropipette aspiration assay to examine calcium mobilization caused by red blood cell mechanosensing.European biophysics journal : EBJ · 2022Article
- Global transcriptional responses of pneumococcus to human blood components and cerebrospinal fluid.Frontiers in microbiology · 2022Article
- Purinergic interplay between erythrocytes and platelets in diabetes-associated vascular dysfunction.Purinergic signalling · 2021Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The hypothesis that regulated ATP release from red blood cells (RBCs) contributes to nitric oxide-dependent control of local blood flow has sparked much interest in underlying release mechanisms. Several stimuli, including shear stress and hypoxia, have been found to induce significant RBC ATP release attributed to activation of ATP-conducting channels. In the present study, we first evaluated different experimental approaches investigating stimulated RBC ATP release and quantifying hemolysis. We then measured ATP and free hemoglobin in each and every RBC supernatant sample to directly assess the contribution of hemolysis to ATP release. Hypotonic shock, shear stress, and hypoxia, but not cyclic adenosine monophosphate agonists, significantly enhanced ATP release. It tightly correlated, however, with free hemoglobin in RBC supernatants, indicating that lysis was responsible for most, if not all, ATP release. Luminescence ATP imaging combined with simultaneous infrared cell imaging showed that ATP was released exclusively from lysing cells with no contribution from intact cells. In summary, with all stimuli tested, we found no evidence of regulated ATP release from intact RBCs other than by cell lysis. Such a release mechanism might be physiologically relevant in vivo, eg, during exercise and hypoxia where intravascular hemolysis, predominantly of senescent cells, is augmented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.