ArticlePlant cell reports2014
A comparison study of Agrobacterium-mediated transformation methods for root-specific promoter analysis in soybean.
Article in Plant cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- GmAP2 enhances plant tolerance to aluminum toxicity and phosphorus deficiency in Arabidopsis.Plant cell reports · 2025Article
- Overexpression of the Wild Soybean Expansin GenePlants (Basel, Switzerland) · 2025Article
- Overexpression of Wild Soybean Expansin GenePlants (Basel, Switzerland) · 2024Article
- Combination of Hairy Root and Whole-Plant Transformation Protocols to Achieve Efficient CRISPR/Cas9 Genome Editing in Soybean.Plants (Basel, Switzerland) · 2023Article
- An EfficientInternational journal of molecular sciences · 2022Article
- Genome-Wide Identification of Expansin Genes in Wild Soybean (International journal of molecular sciences · 2022Article
- Progress in Soybean Genetic Transformation Over the Last Decade.Frontiers in plant science · 2022Review
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4 authors.
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Abstract
key messageBoth in vitro and in vivo hairy root transformation systems could not replace whole plant transformation for promoter analysis of root-specific and low-P induced genes in soybean. An efficient genetic transformation system is crucial for promoter analysis in plants. Agrobacterium-mediated transformation is the most popular method to produce transgenic hairy roots or plants. In the present study, first, we compared the two different Agrobacterium rhizogenes-mediated hairy root transformation methods using either constitutive CaMV35S or the promoters of root-preferential genes, GmEXPB2 and GmPAP21, in soybean, and found the efficiency of in vitro hairy root transformation was significantly higher than that of in vivo transformation. We compared Agrobacterium rhizogenes-mediated hairy root and Agrobacterium tumefaciens-mediated whole plant transformation systems. The results showed that low-phosphorous (P) inducible GmEXPB2 and GmPAP21 promoters could not induce the increased expression of the GUS reporter gene under low P stress in both in vivo and in vitro transgenic hairy roots. Conversely, GUS activity of GmPAP21 promoter was significantly higher at low P than high P in whole plant transformation. Therefore, both in vitro and in vivo hairy root transformation systems could not replace whole plant transformation for promoter analysis of root-specific and low-P induced genes in soybean.
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