ArticleThe Journal of general virology2014
Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers.
Article in The Journal of general virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Interactions between the 2C protein of foot-and-mouth disease virus and components of the viral replication machinery are mediated by endoplasmic reticulum-derived membranes.The Journal of general virology · 2026Article
- Interactions between the 2C protein of FMDV and components of the viral replication machinery are mediated by ER-derived membranes.bioRxiv : the preprint server for biology · 2025Article
- Aptamer-Based Detection of Foot-and-Mouth Disease Virus Using Single-Stranded DNA Probe.Applied biochemistry and biotechnology · 2025Article
- Higher-order structures of the foot-and-mouth disease virus RNA-dependent RNA polymerase required for genome replication.Communications biology · 2022Article
- Aptamers in Virology-A Consolidated Review of the Most Recent Advancements in Diagnosis and Therapy.Pharmaceutics · 2021Review
- Potential applications of aptamers in veterinary science.Veterinary research · 2021Review
- Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach.PloS one · 2021Article
- Genetic economy in picornaviruses: Foot-and-mouth disease virus replication exploits alternative precursor cleavage pathways.PLoS pathogens · 2017Article
- Foot-and-mouth disease virus genome replication is unaffected by inhibition of type III phosphatidylinositol-4-kinases.The Journal of general virology · 2016Article
- Both cis and trans Activities of Foot-and-Mouth Disease Virus 3D Polymerase Are Essential for Viral RNA Replication.Journal of virology · 2016Article
- Innate Immunity and Immune Evasion by Enterovirus 71.Viruses · 2015Review
- Article
- A novel method for the measurement of hepatitis C virus infectious titres using the IncuCyte ZOOM and its application to antiviral screening.Journal of virological methods · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
We have previously documented the inhibitory activity of RNA aptamers to the RNA-dependent RNA polymerase of foot-and-mouth disease virus (3D(pol)). Here we report their modification and use with a subgenomic replicon incorporating GFP (pGFP-PAC replicon), allowing replication to be monitored and quantified in real-time. GFP expression in transfected BHK-21 cells reached a maximum at approximately 8 h post-transfection, at which time change in morphology of the cells was consistent with a virus-induced cytopathic effect. However, transfection of replicon-bearing cells with a 3D(pol) aptamer RNA resulted in inhibition of GFP expression and maintenance of normal cell morphology, whereas a control aptamer RNA had little effect. The inhibition was correlated with a reduction in 3D(pol) (detected by immunoblotting) and shown to be dose dependent. The 3D(pol) aptamers appeared to be more effective than 2'-C-methylcytidine (2'CMC). Aptamers to components of the replication complex are therefore useful molecular tools for studying viral replication and also have potential as diagnostic molecules in the future.
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Registered trials
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