ArticleScientific reports2014
High-throughput monitoring of major cell functions by means of lensfree video microscopy.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 73 citations in OpenAlex.
- Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform.Analytical chemistry · 2025Article
- Chip-Sized Lensless Holographic Microscope for Real-Time On-Chip Biological Sensing.Sensors (Basel, Switzerland) · 2025Article
- Lens-free on-chip 3D microscopy based on wavelength-scanning Fourier ptychographic diffraction tomography.Light, science & applications · 2024Article
- Noninvasive total counting of cultured cells using a home-use scanner with a pattern sheet.iScience · 2024Article
- A novel feature for monitoring the enzymatic harvesting process of adherent cell cultures based on lens-free imaging.Scientific reports · 2022Article
- Review
- Contact guidance as a consequence of coupled morphological evolution and motility of adherent cells.Biomechanics and modeling in mechanobiology · 2022Article
- Review
- TheRoyal Society open science · 2022Article
- Article
- High-Speed Lens-Free Holographic Sensing of Protein Molecules Using Quantitative Agglutination Assays.ACS sensors · 2021Article
- Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy.Scientific reports · 2021Article
- Low-cost calcium fluorometry for long-term nanoparticle studies in living cells.Scientific reports · 2020Article
- Lens-Free Imaging as a Sensor for Dynamic Cell Viability Detection Using the Neutral Red Uptake Assay.ACS applied bio materials · 2020Article
- Biophysical investigation of living monocytes in flow by collaborative coherent imaging techniques.Biomedical optics express · 2018Article
- Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture.Journal of visualized experiments : JoVE · 2018Article
- On-chip Microscopy Using Random Phase Mask Scheme.Scientific reports · 2017Article
- Flatbed epi relief-contrast cellular monitoring system for stable cell culture.Scientific reports · 2017Article
- Time-lapse lens-free imaging of cell migration in diverse physical microenvironments.Lab on a chip · 2016Article
- The Point-of-Care Laboratory in Clinical Microbiology.Clinical microbiology reviews · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantification of basic cell functions is a preliminary step to understand complex cellular mechanisms, for e.g., to test compatibility of biomaterials, to assess the effectiveness of drugs and siRNAs, and to control cell behavior. However, commonly used quantification methods are label-dependent, and end-point assays. As an alternative, using our lensfree video microscopy platform to perform high-throughput real-time monitoring of cell culture, we introduce specifically devised metrics that are capable of non-invasive quantification of cell functions such as cell-substrate adhesion, cell spreading, cell division, cell division orientation and cell death. Unlike existing methods, our platform and associated metrics embrace entire population of thousands of cells whilst monitoring the fate of every single cell within the population. This results in a high content description of cell functions that typically contains 25,000 - 900,000 measurements per experiment depending on cell density and period of observation. As proof of concept, we monitored cell-substrate adhesion and spreading kinetics of human Mesenchymal Stem Cells (hMSCs) and primary human fibroblasts, we determined the cell division orientation of hMSCs, and we observed the effect of transfection of siCellDeath (siRNA known to induce cell death) on hMSCs and human Osteo Sarcoma (U2OS) Cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.