ArticleStem cell reviews and reports2015
Genome-wide demethylation by 5-aza-2'-deoxycytidine alters the cell fate of stem/progenitor cells.
Article in Stem cell reviews and reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 22 citations in OpenAlex.
- Disinherit your Descendants by Rewriting the Chronic Pulmonary Obstructive Disease Epigenetic Script.American journal of respiratory cell and molecular biology · 2024Article
- Single-cell transcriptomic atlas of mouse cochlear aging.Protein & cell · 2023Article
- Inhibiting DNA methylation alleviates cisplatin-induced hearing loss by decreasing oxidative stress-induced mitochondria-dependent apoptosisActa pharmaceutica Sinica. B · 2022Article
- The DNA methylation inhibitor RG108 protects against noise-induced hearing loss.Cell biology and toxicology · 2021Article
- Generation of Cochlear Hair Cells from Sox2 Positive Supporting Cells via DNA Demethylation.International journal of molecular sciences · 2020Article
- DNMT1-mediated Foxp3 gene promoter hypermethylation involved in immune dysfunction caused by arsenic in human lymphocytes.Toxicology research · 2020Article
- Epigenetic regulation of COX‑2 expression by DNA hypomethylation via NF‑κB activation in ketamine‑induced ulcerative cystitis.International journal of molecular medicine · 2019Article
- Identification of Neural Stem Cells from Postnatal Mouse Auditory Cortex In Vitro.Stem cells and development · 2019Article
- Approaches for the study of epigenetic modifications in the inner ear and related tissues.Hearing research · 2019Review
- Applications of Lgr5-Positive Cochlear Progenitors (LCPs) to the Study of Hair Cell Differentiation.Frontiers in cell and developmental biology · 2019Article
- TET1 regulates fibroblast growth factor 8 transcription in gonadotropin releasing hormone neurons.PloS one · 2019Article
- Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos.The Journal of reproduction and development · 2016Article
- Epigenetic DNA Demethylation Causes Inner Ear Stem Cell Differentiation into Hair Cell-Like Cells.Frontiers in cellular neuroscience · 2016Article
- Fibroblast Growth Factor 8 Expression in GT1-7 GnRH-Secreting Neurons Is Androgen-Independent, but Can Be Upregulated by the Inhibition of DNA Methyltransferases.Frontiers in cell and developmental biology · 2016Article
- DNA Methylation Landscapes of Human Fetal Development.PLoS genetics · 2015Article
- 5-Azacitidine induces demethylation of PTPL1 and inhibits growth in non-Hodgkin lymphoma.International journal of molecular medicine · 2015Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
DNA methyltransferase (DNMT) inhibitor 5-aza-2'-deoxycytidine (5-aza-CdR) is able to cause DNA demethylation in the genome and induce the expression of silenced genes. Whether DNA demethylation can affect the gene expression of stem/progenitor cells has not been understood. Mouse utricle epithelia-derived progenitor cells (MUCs), which possess stem cell features as previously described, exhibit a potential DNA methylation status in the genome. In this study, MUCs were treated with 5-aza-CdR to determine whether DNMT inhibitor is able to induce the differentiation of MUCs. With 5-aza-CdR treatment for 72 hr, MUCs expressed epithelial genes including Cdh1, Krt8, Krt18, and Dsp. Further, hair cell genes Myo7a and Myo6 increased their expressions in response to 5-aza-CdR treatment. The decrease in the global methylated DNA values after 5-aza-CdR treatment indicated a significant DNA demethylation in the genome of MUCs, which may contribute to remarkably increased expression of epithelial genes and hair cell genes. The progenitor MUCs then turned into an epithelial-like hair cell fate with the expression of both epithelial and hair cell genes. This study suggests that stem cell differentiation can be stimulated by DNA demethylation, which may open avenues for studying stem cell fate induction using epigenetic approaches.
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