ArticleParasitology research2014
Cloning, expression and characterisation of a type II cystatin from Schistosoma japonicum, which could regulate macrophage activation.
Article in Parasitology research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Neutrophilic granule protein promotes lipopolysaccharide-induced iNOS/NO expression via JAK2/STAT1 signaling pathway and augments bacteria clearance of macrophages.Cellular and molecular life sciences : CMLS · 2025Article
- Cystatin from the helminthFrontiers in immunology · 2024Article
- Type I Cystatin Derived fromPathogens (Basel, Switzerland) · 2023Article
- Article
- Molecular Characteristics and Potent Immunomodulatory Activity of Fasciola hepatica Cystatin.The Korean journal of parasitology · 2022Article
- Tumor hijacks macrophages and microbiota through extracellular vesicles.Exploration (Beijing, China) · 2022Review
- Article
- Immunomics-Guided Antigen Discovery for Praziquantel-Induced Vaccination in Urogenital Human Schistosomiasis.Frontiers in immunology · 2021Article
- Parasite Cystatin: Immunomodulatory Molecule with Therapeutic Activity against Immune Mediated Disorders.Pathogens (Basel, Switzerland) · 2020Review
- Therapeutic efficacy of Schistosoma japonicum cystatin on sepsis-induced cardiomyopathy in a mouse model.Parasites & vectors · 2020Article
- Characterization of mRNA Profiles of Exosomes from Diverse Forms of M2 Macrophages.BioMed research international · 2020Article
- Amelioration of type 1 diabetes by recombinant fructose-1,6-bisphosphate aldolase and cystatin derived from Schistosoma japonicum in a murine model.Parasitology research · 2020Article
- The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin.Cellular and molecular life sciences : CMLS · 2019Article
- Qualitative and quantitative proteomic analyses of Schistosoma japonicum eggs and egg-derived secretory-excretory proteins.Parasites & vectors · 2019Article
- Molecular Characterization of aGenes · 2019Article
- [Intervention with Schistosoma japonicum cysteine protease inhibitor for treatment of lipopolysaccharide-induced sepsis in mice].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
- Therapeutic effect of Schistosoma japonicum cystatin on bacterial sepsis in mice.Parasites & vectors · 2017Article
- Cysteine protease inhibitor of Schistosoma japonicum - A parasite-derived negative immunoregulatory factor.Parasitology research · 2017Article
- Therapeutic potential of recombinant cystatin from Schistosoma japonicum in TNBS-induced experimental colitis of mice.Parasites & vectors · 2016Article
- Exosome-like vesicles derived by Schistosoma japonicum adult worms mediates M1 type immune- activity of macrophage.Parasitology research · 2015Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cystatin play an important role in parasite immune evasion. It is involved in many immune responses processes regulations such as inhibiting antigen presentation, modifying cytokines production and macrophage polarization. In recent years, more and more cystatins were used in treating some inflammatory diseases such as asthma and inflammation bowel diseases; however, cystatins from Schistosoma japonicum were rarely studied. In the present study, we have cloned a cystatin from the adult stage of Schistosoma japonicum, named as SjCystatin, and its sequence shares conserved domains with other type II family cystatins. It was further verified by enzyme inhibition assays. SjCystatin retained its inhibitory activity under a wide range of pH values and temperatures, can maintain its inhibitory activity at pH 6.5-7.5 and 37 °C, respectively. Then, we investigated the effects of SjCystatin on the lipopolysaccharide (LPS)-induced activated RAW264.7. Results showed that SjCystatin inhibit LPS-induced nitric oxide production in a dose-dependent manner. LPS-induced TNF-α and IL-6 production began to be inhibited at least 6 h after SjCystatin stimulation. SjCystatin significantly increased IL-10 production at 6 h after stimulation and its effect on IL-10 production diminished quickly. These results imply that SjCystatin can induce M2 macrophage polarization and can be expected to serve as a potential drug source for the medication of inflammatory disorders like other cystatins.
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