ArticleGenome research2014
Cell fate inclination within 2-cell and 4-cell mouse embryos revealed by single-cell RNA sequencing.
Article in Genome research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 130 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
130 citing papers in PubMed, 1 synthesis or guideline pooled it, 346 citations in OpenAlex.
- Cross-species meta-analysis of transcriptome changes during the morula-to-blastocyst transition: metabolic and physiological changes take center stage.American journal of physiology. Cell physiology · 2021Pooled it
- Single-cell marker gene clustering: A unified deep learning framework for marker gene-based clustering of single-cell RNA-sequencing data.Quantitative biology (Beijing, China) · 2026Article
- Asymmetric RBBP7 regulates the first cell fate decision of early mammalian embryos.Cell discovery · 2026Article
- Subcellularly Resolved 3D Translatome in Mouse Oocytes and Early Embryos.bioRxiv : the preprint server for biology · 2026Article
- Early transcriptional divergence underlies cell fate bias in bovine embryos.Communications biology · 2026Article
- Distinct roles for SETα and SETβ in early cell fate decisions.Nucleic acids research · 2026Article
- Article
- Induction of Stress Granules and Developmental Instability of Offspring Phenotype Due to Hypothermia During First Mouse Embryo Cleavage.International journal of molecular sciences · 2025Article
- Differentiable graph clustering with structural grouping for single-cell RNA-seq data.Bioinformatics (Oxford, England) · 2025Article
- CeiTEA: Adaptive Hierarchy of Single Cells with Topological Entropy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- scHNTL: single-cell RNA-seq data clustering augmented by high-order neighbors and triplet loss.Bioinformatics (Oxford, England) · 2025Article
- Animal and vegetal materials of mouse oocytes segregate at first zygotic cleavage: a simple mechanism that makes the two-cell blastomeres differ reciprocally from the start.Molecular human reproduction · 2025Article
- Topology entropy: Enhancing graph partitioning for TAD identification and single-cell clustering.Computational and structural biotechnology journal · 2025Article
- Constructing Cell-Specific Causal Networks of Individual Cells for Depicting Dynamical Biological Processes.Research (Washington, D.C.) · 2025Article
- scCCTR: An iterative selection-based semi-supervised clustering model for single-cell RNA-seq data.Computational and structural biotechnology journal · 2025Article
- Deep learning-based models for preimplantation mouse and human embryos based on single-cell RNA sequencing.Nature methods · 2025Article
- scVAG: Unified single-cell clustering via variational-autoencoder integration with Graph Attention Autoencoder.Heliyon · 2024Article
- scQA: A dual-perspective cell type identification model for single cell transcriptome data.Computational and structural biotechnology journal · 2024Article
- Antagonism between H3K27me3 and genome-lamina association drives atypical spatial genome organization in the totipotent embryo.Nature genetics · 2024Article
70 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
It remains an open question when and how the first cell fate decision is made in mammals. Using deep single-cell RNA-seq of matched sister blastomeres, we report highly reproducible inter-blastomere differences among 10 2-cell and five 4-cell mouse embryos. Inter-blastomere gene expression differences dominated between-embryo differences and noise, and were sufficient to cluster sister blastomeres into distinct groups. Dozens of protein-coding genes exhibited reproducible bimodal expression in sister blastomeres, which cannot be explained by random fluctuations. The protein expression of one gene out of four of these bimodal genes tested, Gadd45a, exhibited clear inter-blastomeric contrasts. We traced some of the bimodal mRNA expressions to embryonic genome activation, and others to blastomere-specific RNA depletion. Inter-blastomere differences created coexpression gene networks that were much stronger and larger than those that can possibly be created by random noise. The highly correlated gene pairs at the 4-cell stage overlapped with those showing the same directions of differential expression between inner cell mass (ICM) and trophectoderm (TE). These data substantiate the hypothesis of inter-blastomere differences in 2- and 4-cell mouse embryos, and associate these differences with ICM/TE differences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.