ArticleDevelopmental biology2014
Steroid signaling promotes stem cell maintenance in the Drosophila testis.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Sex-biased expression of enteroendocrine cell-derived hormones contributes to higher fat storage ineLife · 2026Article
- Post-transcriptional suppression of the pioneer factor Zelda protects the adult Drosophila testis from activation of the ovary program.PLoS biology · 2025Article
- Mating-induced Ecdysone in the testis disrupts soma-germline contacts and stem cell cytokinesis.Development (Cambridge, England) · 2024Article
- Mating-induced ecdysone in the testis disrupts soma-germline contacts and stem cell cytokinesis.bioRxiv : the preprint server for biology · 2023Article
- Old and newly synthesized histones are asymmetrically distributed in Drosophila intestinal stem cell divisions.EMBO reports · 2023Article
- Review
- Epigenetic regulation of drosophila germline stem cell maintenance and differentiation.Developmental biology · 2021Review
- Orphan nuclear receptor ftz-f1 (NR5A3) promotes egg chamber survival in the Drosophila ovary.G3 (Bethesda, Md.) · 2021Article
- Nuclear receptors linking physiology and germline stem cells in Drosophila.Vitamins and hormones · 2021Review
- Single-cyst transcriptome analysis ofDevelopment (Cambridge, England) · 2020Article
- Transcriptomic analysis of the testicular fusion in Spodoptera litura.BMC genomics · 2020Article
- Mating induces switch from hormone-dependent to hormone-independent steroid receptor-mediated growth in Drosophila secondary cells.PLoS biology · 2019Article
- Review
- RNA-Seq analysis of Polyrhachis vicina Roger and insights into the heat shock protein 90 and 70 families.Cell stress & chaperones · 2019Article
- Protecting and Diversifying the Germline.Genetics · 2018Review
- Relevance of estrogen-related receptor gene and ecdysone receptor gene in adult testis of the cricket Teleogryllus emma (Orthoptera: Gryllidae).Die Naturwissenschaften · 2017Article
- Steroid Hormones and the Physiological Regulation of Tissue-Resident Stem Cells: Lessons from theCurrent stem cell reports · 2017Article
- Enhancer of polycomb coordinates multiple signaling pathways to promote both cyst and germline stem cell differentiation in the Drosophila adult testis.PLoS genetics · 2017Article
- CONSERVED AND EXAPTED FUNCTIONS OF NUCLEAR RECEPTORS IN ANIMAL DEVELOPMENT.Nuclear receptor research · 2017Article
- Transcriptome Analysis and Differential Gene Expression on the Testis of Orange Mud Crab, Scylla olivacea, during Sexual Maturation.PloS one · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Stem cell regulation by local signals is intensely studied, but less is known about the effects of hormonal signals on stem cells. In Drosophila, the primary steroid twenty-hydroxyecdysone (20E) regulates ovarian germline stem cells (GSCs) but was considered dispensable for testis GSC maintenance. Male GSCs reside in a microenvironment (niche) generated by somatic hub cells and adjacent cyst stem cells (CySCs). Here, we show that depletion of 20E from adult males by overexpressing a dominant negative form of the Ecdysone receptor (EcR) or its heterodimeric partner ultraspiracle (usp) causes GSC and CySC loss that is rescued by 20E feeding, uncovering a requirement for 20E in stem cell maintenance. EcR and USP are expressed, activated and autonomously required in the CySC lineage to promote CySC maintenance, as are downstream genes ftz-f1 and E75. In contrast, GSCs non-autonomously require ecdysone signaling. Global inactivation of EcR increases cell death in the testis that is rescued by expression of EcR-B2 in the CySC lineage, indicating that ecdysone signaling supports stem cell viability primarily through a specific receptor isoform. Finally, EcR genetically interacts with the NURF chromatin-remodeling complex, which we previously showed maintains CySCs. Thus, although 20E levels are lower in males than females, ecdysone signaling acts through distinct cell types and effectors to ensure both ovarian and testis stem cell maintenance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.