ReviewTransgenic research2014
The new CRISPR-Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.
Review in Transgenic research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 76 citations in OpenAlex.
- Generation of a Ym1 deficient mouse utilising CRISPR-Cas9 in CB6 embryos.Transgenic research · 2025Article
- Genetic susceptibility to diabetic kidney disease is linked to promoter variants of XOR.Nature metabolism · 2023Article
- Transgenesis and Genome Engineering: A Historical Review.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective.Microorganisms · 2022Review
- Historical DNA Manipulation Overview.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Boundary sequences flanking the mouse tyrosinase locus ensure faithful pattern of gene expression.Scientific reports · 2020Article
- Inhibiting MAPK14 showed anti-prolactinoma effect.BMC endocrine disorders · 2020Article
- Article
- A history of genome editing in mammals.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Contributions of Mammalian Chimeras to Pluripotent Stem Cell Research.Cell stem cell · 2016Review
- Gene targeting, genome editing: from Dolly to editors.Transgenic research · 2016Review
- Animal models of pituitary neoplasia.Molecular and cellular endocrinology · 2016Review
- Review
- The role of genetically engineered pigs in xenotransplantation research.The Journal of pathology · 2016Review
- CRISPR-Cas9 directed knock-out of a constitutively expressed gene using lance array nanoinjection.SpringerPlus · 2016Article
- A Rapid and Cheap Methodology for CRISPR/Cas9 Zebrafish Mutant Screening.Molecular biotechnology · 2016Article
- Engineering large animal models of human disease.The Journal of pathology · 2016Review
- Advances in the Generation of Genetically Modified (GM) Animal Models: Meeting report.Transgenic research · 2015Article
- CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR-Cas system is the newest targeted nuclease for genome engineering. In less than 1 year, the ease, robustness and efficiency of this method have facilitated an immense range of genetic modifications in most model organisms. Full and conditional gene knock-outs, knock-ins, large chromosomal deletions and subtle mutations can be obtained using combinations of clustered regularly interspaced short palindromic repeats (CRISPRs) and DNA donors. In addition, with CRISPR-Cas compounds, multiple genetic modifications can be introduced seamlessly in a single step. CRISPR-Cas not only brings genome engineering capacities to species such as rodents and livestock in which the existing toolbox was already large, but has also enabled precise genetic engineering of organisms with difficult-to-edit genomes such as zebrafish, and of technically challenging species such as non-human primates. The CRISPR-Cas system allows generation of targeted mutations in mice, even in laboratories with limited or no access to the complex, time-consuming standard technology using mouse embryonic stem cells. Here we summarize the distinct applications of CRISPR-Cas technology for obtaining a variety of genetic modifications in different model organisms, underlining their advantages and limitations relative to other genome editing nucleases. We will guide the reader through the many publications that have seen the light in the first year of CRISPR-Cas technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.