Evidence map›Paper›PMID 25092091›Full record

ArticleJournal of pediatric surgery2014

Induction of chemokine (C-C motif) ligand 2 by sphingosine-1-phosphate signaling in neuroblastoma.

Mei-Hong Li, Miriam Harel, Timothy Hla, Fernando Ferrer

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pediatric surgery, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Article
  3. Targeting the myeloid microenvironment in neuroblastoma.Journal of experimental & clinical cancer research : CR · 2023
    Review
  4. Article
  5. Review
  6. Decreased Astrocytic CCL2 Accounts for BAF-312 Effect on PBMCs Transendothelial Migration Through a Blood Brain Barrier in Vitro Model.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2022
    Article
  7. Article
  8. Article
  9. Review
  10. Antitumor Activity of a Novel Sphingosine-1-Phosphate 2 Antagonist, AB1, in Neuroblastoma.The Journal of pharmacology and experimental therapeutics · 2015
    Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Mei-Hong LiCenter for Vascular Biology, University of Connecticut Health Center, Farmington, CT 06030. Electronic address: mhli@uchc.edu.
Miriam HarelDepartment of Urology and Surgery, Connecticut Children's Medical Center, Hartford, CT 06106.
Timothy HlaCenter for Vascular Biology, Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10065.
Fernando FerrerCenter for Vascular Biology, University of Connecticut Health Center, Farmington, CT 06030; Department of Urology and Surgery, Connecticut Children's Medical Center, Hartford, CT 06106. Electronic address: fferrer@connecticutchildrens.org.
Connecticut Children's Medical Center · USCornell University · USUConn Health · US

Funding

THE ROLE OF ENDOCANNABINOIDS AND THEIR RECEPTORS IN COLORECTAL CANCERP01CA077839 · NCI · VANDERBILT UNIVERSITY · PI DUBOIS, RAYMOND N. · 1999 to 2016
$17.8M
Sphingosine-1-Phosphate Pathway Based Therapy for NeuroblastomaR01CA168903 · NCI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI FERRER, FERNANDO A, SHAPIRO, LINDA H · 2012 to 2016
$1.7M
NCI NIH HHS P01 CA077839NCI NIH HHS R01 CA168903NCI NIH HHS R01CA168903
6 · The paper itself

Abstract

background/purposeNeuroblastoma (NB) is the most common extracranial solid tumor of childhood. Preliminary data derived from a human angiogenesis array in NB showed that the bioactive lipid sphingosine-1-phosphate (S1P) induced the secretion of several angiogenesis-related proteins including the important inflammatory factor chemokine (C-C motif) ligand 2 (CCL2). In the present study, we investigated the mechanism of S1P-induced CCL2 expression in NB.

methodsQuantitative real-time PCR and CCL2 ELISA were conducted to detect the mRNA expression and protein secretion of CCL2 in NB cells. Gain and loss of function studies were performed by using specific S1PR antagonists, adenoviral transduction and siRNA transfection. Macrophage F4/80 receptor in NB xenografts was detected by quantitative real-time PCR and immunohistochemistry staining.

resultsS1P induced CCL2 mRNA expression and protein secretion in a time- and concentration-dependent manner in NB cells. Blockade of S1P2 signaling using the selective S1P2 antagonist JTE-013 inhibited S1P-induced CCL2 expression. Overexpression of S1P2 by adenoviral transduction increased CCL2 secretion while knockdown of S1P2 by siRNA transfection decreased S1P-induced CCL2 secretion in NB cells. Macrophage infiltration, as detected by F4/80 staining, was significantly decreased in JTE-013-treated NB xenografts.

conclusionsTaken together, our data for the first time demonstrate that S1P induced the macrophage-recruiting factor CCL2 expression in NB cells via S1P2, providing new insights into the complicated functions of S1P2 in cancer.

Indexed as

Gene Expression Regulation, NeoplasticChemokines, CEnzyme-Linked Immunosorbent AssayHumansImmunohistochemistryLysophospholipidsNeuroblastomaReal-Time Polymerase Chain ReactionRNA, NeoplasmSignal TransductionSphingosineTumor Cells, CulturedChemokines, CLysophospholipidsRNA, NeoplasmSphingosinesphingosine 1-phosphateXCL2 protein, humanChemokine (C-C motif) ligand 2NeuroblastomaSphingosine 1-phosphateSphingosine 1-phosphate receptor 2Tumor-associated macrophage

Identifiers

PMID25092091
PMCPMC4122984
OpenAlexW2135512755

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.