ArticleJournal of Zhejiang University. Science. B2014
Piper betle leaf extract enhances the cytotoxicity effect of 5-fluorouracil in inhibiting the growth of HT29 and HCT116 colon cancer cells.
Article in Journal of Zhejiang University. Science. B, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Antimicrobial additives in resin-based endodontic sealers: a scoping review of antimicrobial efficacy, physicochemical properties, and cytotoxicity.Frontiers in dental medicine · 2026Pooled it
- Effect of Ketorolac on Pharmacokinetics and Pharmacodynamics of 5-Fluorouracil: In Vivo and In Vitro Study.Journal of tropical medicine · 2022Article
- Hydroxychavicol, a polyphenol fromJournal of Zhejiang University. Science. B · 2021Article
- Calotropis gigantea stem bark extract induced apoptosis related to ROS and ATP production in colon cancer cells.PloS one · 2021Article
- Medicinal Plants in the Prevention and Treatment of Colon Cancer.Oxidative medicine and cellular longevity · 2019Review
- Combinatorial Cytotoxic Effects of Gelam Honey and 5-Fluorouracil against Human Adenocarcinoma Colon Cancer HT-29 Cells In Vitro.International journal of cell biology · 2019Article
- The Effect of BornylInternational journal of molecular sciences · 2018Article
- Herbal Formulation C168 Attenuates Proliferation and Induces Apoptosis in HCT 116 Human Colorectal Carcinoma Cells: Role of Oxidative Stress and DNA Damage.Evidence-based complementary and alternative medicine : eCAM · 2016Article
- Anti-migratory effects of Piper betle leaf aqueous extract on cancer cells and its microtubule targeting properties.Journal of Zhejiang University. Science. BArticle
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe combination effect of Piper betle (PB) and 5-fluorouracil (5-FU) in enhancing the cytotoxic potential of 5-FU in inhibiting the growth of colon cancer cells was investigated.
methodsHT29 and HCT116 cells were subjected to 5-FU or PB treatment. 5-FU and PB were then combined and their effects on both cell lines were observed after 24 h of treatment. PB-5-FU interaction was elucidated by isobologram analysis. Apoptosis features of the treated cells were revealed by annexin V/PI stain. High-performance liquid chromatography (HPLC) was performed to exclude any possible chemical interaction between the compounds.
resultsIn the presence of PB extract, the cytotoxicity of 5-FU was observed at a lower dose (IC50 12.5 µmol/L) and a shorter time (24 h) in both cell lines. Both cell lines treated with 5-FU or PB alone induced a greater apoptosis effect compared with the combination treatment. Isobologram analysis indicated that PB and 5-FU interacted synergistically and antagonistically in inhibiting the growth of HT29 and HCT116 cells, respectively.
conclusionsIn the presence of PB, a lower dosage of 5-FU is required to achieve the maximum drug effect in inhibiting the growth of HT29 cells. However, PB did not significantly reduce 5-FU dosage in HCT116 cells. Our result showed that this interaction may not solely contribute to the apoptosis pathway.
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