Evidence map›Paper›PMID 25091817›Full record

ArticleGlycobiology2015

RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.

Sayaka Akiyoshi, Kazuko H Nomura, Katsufumi Dejima, Daisuke Murata, Ayako Matsuda, Nanako Kanaki, Tetsuro Takaki, Hiroyuki Mihara, Takayuki Nagaishi, Shuhei Furukawa and 8 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Glycobiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 16 citations in OpenAlex.

  1. Parasitology · 2021
    Article
  2. iScience · 2020
    Article
  3. Article
  4. Article
  5. N-glycomic Complexity in Anatomical Simplicity:Frontiers in molecular biosciences · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 5 institutions in 2 countries.

Sayaka AkiyoshiGraduate School of Systems Life Sciences, and.
Kazuko H NomuraDepartment of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan.
Katsufumi DejimaDepartment of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
Daisuke MurataGraduate School of Systems Life Sciences, and Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan.
Ayako MatsudaGraduate School of Systems Life Sciences, and.
Nanako KanakiDepartment of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
Tetsuro TakakiDepartment of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
Hiroyuki MiharaDepartment of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
Takayuki NagaishiGraduate School of Systems Life Sciences, and Department of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
Shuhei FurukawaDepartment of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan.
Keiko-Gengyo AndoCore Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
Sawako YoshinaDepartment of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
Shohei MitaniCore Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan Department of Physiology, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.
Akira TogayachiGlycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
Yoshinori SuzukiGlycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
Toshihide ShikanaiGlycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
Hisashi NarimatsuGlycomedicine Technology Research Center (GTRC), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8568, Japan.
Kazuya NomuraDepartment of Biological Sciences, Faculty of Sciences 33, Kyushu University, Fukuoka 812-8581, Japan Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Kawaguchi Center Building, 4-1-8 Hon-cho, Kawaguchi, Saitama 332-0012, Japan knomuscb@kyushu-u.org.
Kyushu University · JPLife Systems (United States) · USNational Institute of Advanced Industrial Science and Technology · JPJapan Science and Technology Agency · JPTokyo Women's Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we selected 181 nematode glycogenes that are orthologous to human glycogenes and examined their RNAi phenotypes. The results are deposited in the Caenorhabditis elegans Glycogene Database (CGGDB) at AIST, Tsukuba, Japan. The most prominent RNAi phenotypes observed are disruptions of cell cycle progression in germline mitosis/meiosis and in early embryonic cell mitosis. Along with the previously reported roles of chondroitin proteoglycans, glycosphingolipids and GPI-anchored proteins in cell cycle progression, we show for the first time that the inhibition of the functions of N-glycan synthesis genes (cytoplasmic alg genes) resulted in abnormal germline formation, ER stress and small body size phenotypes. The results provide additional information on the roles of glycoconjugates in the cell cycle progression mechanisms of germline and embryonic cells.

Indexed as

Databases, GeneticGenes, HelminthRNA InterferenceAnimalsBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsCarbohydrate SequenceCell Cycle ProteinsEndoplasmic Reticulum StressGene Expression Regulation, DevelopmentalGenotypeGerm CellsGlycoconjugatesGlycosaminoglycansGlycosphingolipidsCaenorhabditis elegans ProteinsCell Cycle ProteinsGlycoconjugatesGlycosaminoglycansGlycosphingolipidsRNA, Small Interferingalg genesgermline cell divisionglycogeneoligosaccharyltransferaseRNAi

Identifiers

PMID25091817
PMCPMC4245905
OpenAlexW2130057019

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.