Evidence map›Paper›PMID 25091686›Full record

ArticleVaccine2014

Approach to molecular characterization of partially and completely untyped samples in an Indian rotavirus surveillance program.

Sudhir Babji, Rajesh Arumugam, Anuradha Sarvanabhavan, Jon R Gentsch, Gagandeep Kang

Open access · hybridAbstract read
In one paragraph

Article in Vaccine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Persistence of G10P[11] neonatal rotavirus infections in southern India.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Sudhir BabjiDivision of Gastrointestinal Sciences, Christian Medical College, Vellore, India. Electronic address: sudhirbabji79@cmcvellore.ac.in.
Rajesh ArumugamDivision of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
Anuradha SarvanabhavanDivision of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
Jon R GentschCenters for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA.
Gagandeep KangDivision of Gastrointestinal Sciences, Christian Medical College, Vellore, India.
Christian Medical College & Hospital · INCenters for Disease Control and Prevention · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

Surveillance networks for rotavirus document the burden of the disease using the proportion of children hospitalized with gastroenteritis positive for rotavirus by enzyme immunoassay. They also describe genotypes of circulating viruses by polymerase chain reaction for the VP7 and VP4 genes, which determine G and P types, respectively. A proportion of samples cannot be genotyped based on initial testing and laboratories need to assess further testing strategies based on resources and feasibility. To 365 samples obtained from an Indian rotavirus strain surveillance program, we applied an approach to determine the G and P types in antigen positive samples that failed to type initially with the standard laboratory protocol. Fifty-eight samples (19%) were negative for the VP6 gene, indicating that the antigen test was likely to have been false positive. Alternative extraction and priming approaches resulted in the identification of G and P types for 264 strains. The identity of one strain was determined by sequencing the first-round amplicons. Thirty-five strains were partially typed and seven strains could not be typed at all. The distribution of G and P types among strains that had initially failed to type, except one strain, did not differ from that in strains that were typed using the standard laboratory protocol.

Indexed as

GenotypeAntigens, ViralCapsid ProteinsGastroenteritisHumansIndiaMolecular EpidemiologyPopulation SurveillanceRotavirusRotavirus InfectionsSequence Analysis, DNAAntigens, ViralCapsid ProteinsVP4 protein, RotavirusVP7 protein, RotavirusELISA false positiveSpecific primingUntyped Protocol

Identifiers

PMID25091686
PMCPMC7934339
OpenAlexW2083836614

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.